Apoptotic Effect of Cryptotanshinone on Human Melanoma A375 Cells  

作　　者：Jinqian LI Jiaru WANG Lingqi MENG Yi ZHANG Hao WANG Wanting XU Yang LIU Yu ZHANG Shinong WANG Yuqing ZHAI Yinghua LUO Chenghao JIN 

机构地区：[1]College of Life Science & Technology,Heilongjiang Bayi Agricultural University

出　　处：《Medicinal Plant》2018年第6期39-42,共4页药用植物：英文版

基　　金：Supported by Multigrain Production and Processing Characteristic Discipline Construction Project;Postdoctoral Scientific Research Foundation of Heilongjiang Province of China(LBH-Q13132)

摘　　要：[Objectives] The aim was to investigate the effect of cryptotanshinone on apoptosis of human melanoma A375 cells and its related mechanism of mitochondrial pathway.[Methods]The cytotoxic effect of cryptotanshinone on apoptosis of human melanoma A375 cells was detected by MTT colorimetry.The apoptosis of melanoma A375 cells was detected with Annexin V-FITC/PI and observed by fluorescence inverted microscope.The expression of apoptosis-related proteins was detected by Western blotting.[Results]The viability of the A375 cells decreased with the increase of drug concentration.The fluorescence intensity of the cells increased with the treatment time.The expression of pro-apoptotic protein caspase-3 gradually increased,while the expression of apoptosis-inhibiting proteins p-AKT and Bcl-2 gradually reduced.[Conclusions]Cryptotanshinone induces apoptosis of human melanoma A375 cells via AKT signaling pathway,thus exerting a good cytotoxic effect on A375 cells.[Objectives] The aim was to investigate the effect of cryptotanshinone on apoptosis of human melanoma A375 cells and its related mechanism of mitochondrial pathway.[Methods]The cytotoxic effect of cryptotanshinone on apoptosis of human melanoma A375 cells was detected by MTT colorimetry.The apoptosis of melanoma A375 cells was detected with Annexin V-FITC/PI and observed by fluorescence inverted microscope.The expression of apoptosis-related proteins was detected by Western blotting.[Results]The viability of the A375 cells decreased with the increase of drug concentration.The fluorescence intensity of the cells increased with the treatment time.The expression of pro-apoptotic protein caspase-3 gradually increased,while the expression of apoptosis-inhibiting proteins p-AKT and Bcl-2 gradually reduced.[Conclusions]Cryptotanshinone induces apoptosis of human melanoma A375 cells via AKT signaling pathway,thus exerting a good cytotoxic effect on A375 cells.

关 键 词：CRYPTOTANSHINONE Human MELANOMA A375 cells CYTOTOXIC EFFECT Apoptosis AKT signaling pathway 

分 类 号：R[医药卫生]