虾头、虾壳抗氧化肽的分离纯化及其对秀丽隐杆线虫的抗氧化作用  

Puri?cation of Antioxidant Peptides Derived from Enzymatic Hydrolysates of Shrimp Heads and Shells and Their Antioxidant Protection in Caenorhabditis elegans

作　　者：王晋 张风[1] 周爱梅[1] 黄炜超 王淑惠 罗旭洸 WANG Jin;ZHANG Feng;ZHOU Aimei;HUANG Weichao;WANG Shuhui;LUO Xuguang(College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品科学》2019年第3期56-63,共8页Food Science

基　　金：广东省农业科技攻关计划项目(2007A020100006-9)

摘　　要：采用超滤、凝胶过滤色谱和制备液相色谱对南美白对虾虾头、虾壳蛋白质的酶解液进行分离纯化,以1,1-二苯基-2-苦基肼自由基清除率、还原力、氧化自由基吸收能力为指标,制备纯度较高、抗氧化活性较强的抗氧化肽,同时,采用秀丽隐杆线虫(Caenorhabditis elegans)模型对其体内抗氧化活性进行评价。结果表明:酶解液经超滤分离后,分子质量为3～10 kDa组分的抗氧化活性最强;将该组分采用凝胶过滤色谱、制备液相色谱等手段分离出含有抗氧化肽的混合物(抗氧化肽纯度达到70%)。体内抗氧化活性结果显示,喂食该抗氧化肽的秀丽隐杆线虫的产卵量和热应激条件下的存活率均显著增长,寿命显著延长(P<0.05),且与VC相比效果无显著差异(P>0.05),说明本研究所获得的抗氧化肽对秀丽隐杆线虫具有和VC相当的抗氧化保护作用,具有潜在的应用价值。Highly pure peptides with strong antioxidant activity in terms of 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity,reducing power and oxygen radical absorbance capacity(ORAC)were obtained from the enzymatic hydrolysates of heads and shells of Penaeus vannamei by sequential ultrafiltration,gel filtration chromatography and preparative liquid chromatography.Furthermore,Caenorhabditis elegans were used to determine the antioxidant activity in vivo of the obtained antioxidant peptides.The results showed that the ultrafiltration fraction with molecular mass of 3–10 kDa exhibited the highest antioxidant activity.Further purification by gel filtration chromatography and preparative liquid chromatography could obtain a mixture of antioxidant peptides with a purity of 70%.The investigation of antioxidant activity in vivo showed that Caenorhabditis elegans fed with the purified antioxidant peptides laid more eggs and had higher survival rate under heat stress as well as significantly longer lifespan(P<0.05),while the effect was not significantly different from that of VC(P>0.05),indicating that the antioxidant peptides obtained in this study had protective effect against oxidative stress in Caenorhabditis elegans similar to that of VC and therefore could have potential application value.

关 键 词：南美白对虾 虾头、虾壳蛋白质 抗氧化肽 分离纯化 秀丽隐杆线虫 抗氧化活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]