超高压处理僵直前兔肉对其斩拌肉糜流变特性及蛋白二级结构的影响  

Effects of high pressure processing of pre-rigor rabbit muscles on dynamic rheological properties and protein secondary structures of the meat batters

作　　者：薛思雯[1] 衣晓坤 于小波 徐幸莲[1] XUE Siwen;YI Xiaokun;YU Xiaobo;XU Xinglian(Nanjing Agricultural University,College of food science and technology,Key Laboratory ofMeat Processing and Quality Control,MOE/Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control,Nanjing 210095,China;Shangdong Yantai Oriental Technology Co., Ltd, Yantai 246003)

机构地区：[1]南京农业大学食品科学与技术学院肉品加工与质量控制教育部重点实验室江苏省肉类生产与加工质量安全控制协同创新中心,江苏南京210095 [2]山东省烟台市东方海洋科技股份有限公司,山东烟台246003

出　　处：《食品与发酵工业》2019年第6期77-82,共6页Food and Fermentation Industries

基　　金：国家自然科学基金(31471601);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为探究兔肉在僵直前经不同的超高压处理,对其斩拌肉糜在加热后(20～80℃)所形成的凝胶品质的影响。实验以兔肉为主要原料,将僵直前的兔里脊肉在100～300 MPa下分别保压15 s或180 s,再成熟,加盐(质量分数2%)斩拌。对肉糜的储能模量(G')和相位角等流变特性进行测定和分析,同时测定肉糜的拉曼光谱,计算蛋白的二级结构变化情况。结果表明,对僵直前的兔肉进行超高压处理,会影响其斩拌后蛋白体系中蛋白质的空间构型和理化性质,总体上会使α-螺旋含量降低,β-折叠含量增加,其中以200 MPa-15 s处理组最为显著。这些变化与加热后形成的凝胶功能特性有显著的相关性。This study aimed to explore the effects of applying different high pressure( HP) treatments to the prerigor rabbit meat( PRRM) on the gelling property of its meat batters after heating(20-80 ℃). Rabbit meat was used as raw material,PRRM was subjected to 100-300 MPa HP for 15 s and 180 s,respectively,before aging. The aged meat was chopped with 2% salt. The storage modulus( G’),phase angle,and other rheological properties of meat batters were analyzed. Meanwhile,the Raman spectra were determined and the secondary structural changes of proteins were calculated. The results showed that applying HP treatments to PRRM affected conformations and physicochemical properties of proteins in the batter system,as the content of α-helix reduced and the content of β-sheet enhanced. Samples treated by 200 MPa HP for 15 s exhibited the most significant modifications. These changes had significant correlations with the gelling property of meat batters after heating.

关 键 词：兔肉 僵直前 超高压处理 二级结构 流变特性 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]