核酸适配体在食源性致病菌检测中应用的研究进展  

Research Progress on Utilization of Nucleic Acid Aptamer in Detection for Foodborne Pathogenic Bacteria

作　　者：胡金强[1,2,3,4] 黄润娜 王一 赵卫东 高辉 姜春鹏[1] 耿尧[1] 景建洲[1] 董彩文[1] 王章存[1] HU Jin-qiang;HUANG Run-na;WANG Yi;ZHAO Wei-dong;GAO Hui;JIANG Chun-peng;GENG Yao;JING Jian-zhou;DONG Cai-wen;WANG Zhang-cun(School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450000,China;International Joint Laboratory of Food Safety,Zhengzhou 450000,China;Collaborative Innovation Center of Food Production and Safety,Zhengzhou 450000,China;Henan Key Laboratory of Cold Chain Food Quality and Safety Control,Zhengzhou 450000,China)

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450000 [2]河南省食品安全国际联合实验室,河南郑州450000 [3]食品生产与安全河南省协同创新中心,河南郑州450000 [4]河南省冷链食品质量安全控制重点实验室,河南郑州450000

出　　处：《食品工业科技》2019年第9期315-322,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(31201901);河南省自然科学基金(182300410096);河南省科技计划项目(182102310069);河南省高校青年骨干教师资助计划项目(2015GGJS-182);河南省教育厅科学技术重点研究项目(14A180025);郑州轻工业学院青年骨干教师资助计划项目(2013QNGG02);食品生产与安全河南省协同创新中心研究生创新基金项目(FCICY201614)

摘　　要：核酸适配体(aptamer)是经体外筛选可特异性识别并结合靶分子的寡核苷酸片段(单链DNA/RNA序列),具有高特异性、高灵敏度、高亲和力及易于修饰等优点,已在食源性致病菌检测领域得到应用。本文主要对核酸适配体筛选技术分类以及纳米金适配体技术、荧光适配体技术、电化学适配体技术、流式细胞适配体技术和表面增强拉曼适配体技术在食源性致病菌检测中的应用作简要综述。The aptamer is an oligonucleotide fragment(single-stranded DNA/RNA sequence)which can specifically recognize and bind to target molecules by screening in vitro.It has the obvious advantages of high specificity and sensitivity,high affinity and simplicity of modification,and has been applied in the field of foodborne pathogenic bacteria detection.The classification of aptamer screening technologies and nanogold aptamer technology,fluorescence aptamer technology,electrochemical aptamer technology,flow cytometry technology and surface enhanced Raman aptamer technology in the detection of foodborne pathogenic bacteria were mainly reviewed.

关 键 词：食源性致病菌 适配体 检测 

分 类 号：TS201.1[轻工技术与工程—食品科学]