朝鲜辣白菜中抗扩展青霉乳酸菌的筛选与鉴定  

Screening and identification of lactic acid bacterium against Penicillium expansum from kimchi

作　　者：孙悦 刘佳伊 杜宏 李婧 白凤翎 王君勇 励建荣 SUN Yue;LIU Jiayi;DU Hong;LI Jing;BAI Fengling;WANG Junyong;LI Jianrong(National & Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Food Safety Key Lab of Liaoning Province,College of Food Science and Engineering,Bohai University,Jinzhou 121013,China;The Inspection and Quarantine Technology Center of Ningxia Entry-Exit Inspection and Quarantine Bureau,Yinchuan 750000,China)

机构地区：[1]渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]宁夏出入境检验检疫局检验检疫综合技术中心,宁夏银川750000

出　　处：《中国酿造》2019年第6期64-68,共5页China Brewing

基　　金：辽宁省高等学校生鲜食品产业技术研究院支持项目[辽教办发(2017)55号]

摘　　要：该研究采用菌饼法从朝鲜辣白菜中筛选抗扩展青霉(Penicillium expansum)的乳酸菌,采用形态观察、生理生化试验及分子生物学技术进行菌种鉴定,并对乳酸菌抗扩展青霉的机理进行初步研究。结果表明,经过筛选和鉴定,获得1株对扩展青霉抑制效果最佳的清酒乳杆菌(Lactobacillus sakei)PC-3,抑菌率为68.62%。菌株PC-3无细胞上清液(CFS)的抗真菌活性随pH的升高呈现先升高后降低的趋势;随温度的升高逐渐下降。初步判定菌株PC-3 CFS中抗扩展青霉的有效成分是乳酸和乙酸,其对扩展青霉抑菌率分别为67.58%和66.73%。经扫描电镜观察发现,菌株PC-3 CFS抗扩展青霉的机制为破坏其细胞结构,导致胞内物质泄漏。A lactic acid bacterium against Penicillium expansum was screened from kimchi by fungus cake method, and then identified by morphological observation, physiological and biochemical tests and molecular biological technologies, and the mechanism of lactic acid bacteria against P. expansum was researched primarily. The results showed that one strain of Lactobacillus sakei PC-3 with the optimal inhibitory effect on P. expansum was obtained by screening and identification, and the antimycotic rate was 68.62%. The antimycotic activity of cell-free supernatants (CFS) of strain PC-3 increased first and then decreased with the increase of pH, and decreased with the increase of temperature. It was preliminary determined that lactic acid and acetic acid of strain PC-3 CFS were the effective components against P. expansum, and the antimycotic rates were 67.58% and 66.73%, respectively. The scanning electron microscope images revealed that cell structure of P. expansum was damaged by the CFS of strain PC-3, resulting in the leakage of intracellular materials.

关 键 词：朝鲜辣白菜 乳酸菌 扩展青霉 抗真菌活性 筛选与鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]