毛细管电泳技术检测羊乳婴幼儿配方粉中的牛乳成分  

Detection of Cow Milk Components in Goat Milk from Infant Formula Using Capillary Gel Electrophoresis Method

作　　者：刘鸣畅[1] 侯艳梅 杨艳歌[1] 黄文胜[1] 陈颖[1] 吴亚君[1] Liu Mingchang;Hou Yanmei;Yang Yange;Huang Wensheng;Chen Ying;Wu Yajun(Chinese Academy of Inspection and Quarantine,Beijing 100176;Hyproca nutrition (China) Co.Ltd.,Changsha 410005)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]海普诺凯营养品有限公司,长沙410005

出　　处：《中国食品学报》2019年第5期270-275,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家863计划项目(2013AA102202)

摘　　要：婴幼儿配方乳粉的乳源鉴别对于保障产品安全和真实性至关重要。采用毛细管电泳技术对羊乳来源的婴幼儿配方粉中的牛乳成分进行检测。通过比较不同乳源产品中主要蛋白质组分的电泳图谱,筛选出牛乳表征蛋白峰。通过方法验证以及检测市售样品,证实毛细管凝胶电泳在准确度、精密度及重复性方面表现良好,筛选的表征蛋白峰峰面积与羊乳中牛乳含量呈现良好线性相关性(R^2>0.99),检测灵敏度0.5%,表明该方法可用于羊乳来源婴幼儿配方粉中牛乳成分的定性和定量检测。Characterization of milk source is of dramatic importance to ensure the safety and authenticity of infant formula milk.In this study,capillary gel electrophoresis(CGE)method was applied to detect cow milk protein ingredient in goat milk from infant formula.Through comparison of the electrophoresis profiles of protein constituent from different milk source,cow milk specific protein peak was determined.Subsequent validation experiments and testing of commercial infant formula milk confirmed good performance of CGE method in terms of specificity,sensitivity and reproducibility.Area of the specific protein peak showed good linear correlation with the ratio of spiked cow milk to goat milk(R^2>0.99)with a detection limit around 0.5%.These data proved that CGE method is promising in both qualitative and quantitative detection of cow milk in goat milk derived infant formula.

关 键 词：毛细管凝胶电泳 羊乳婴幼儿配方粉 牛乳 蛋白 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程] O657.8[轻工技术与工程—食品科学与工程]