蜂蜜接合酵母对营养物质利用及其高糖胁迫应激代谢特性分析  

Nutrient Utilization of Zygosaccharomyces mellis and Its Metabolic Characteristics in Response to High-Glucose Stress

作　　者：刘功良[1] 费永涛 余洁瑜 刘锐[1] 高苏娟[1] 白卫东[1] LIU Gongliang;FEI Yongtao;YU Jieyu;LIU Rui;GAO Sujuan;BAI Weidong(College of Light Industry and Food Science,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院轻工食品学院

出　　处：《食品科学》2019年第14期166-171,共6页Food Science

基　　金：广东省科技创新战略专项(2018B020206001);“十三五”国家重点研发计划重点专项(2018YFC1604105);2019年广东省农业科技创新及推广项目(2019KJ101)

摘　　要：研究6株蜂蜜接合酵母利用碳源、氮源和维生素的能力,发现蜂蜜接合酵母6-7431对营养物质的代谢能力强于其他菌株,具有较好应用潜力。进一步利用扫描电镜、高效液相色谱检测等方法对该菌株在高糖胁迫下的细胞形态、繁殖方式和应激代谢产物进行探究,结果发现在300~550 g/L的高糖环境中,菌株6-7431能正常出芽生殖,其代谢产生的乙醇、甘油、海藻糖、有机酸(苹果酸、α-酮戊二酸和柠檬酸)等物质含量都远高于100 g/L含糖环境;在550~700 g/L高糖环境中,菌株6-7431的繁殖方式由出芽生殖逐渐变为孢子生殖,代谢产生的乙醇含量低于100 g/L含糖环境,但苹果酸和α-酮戊二酸质量浓度仍有所增加;在750 g/L高糖环境中,菌株6-7431严重失水,形态干瘪,其产生的乙醇体积分数为0.6%,基本停止发酵活动。研究结果揭示了高糖环境对蜂蜜接合酵母细胞形态、繁殖方式以及应激代谢产物的影响,对分析酵母的高糖耐受机制具有重要的参考价值。The abilities of six Zygosaccharomyces mellis strains to metabolize carbon sources, nitrogen sources and vitamins were investigated. It turned out that Z. mellis 6-7431 had stronger ability of utilizing nutrients than the other strains. Then the physiological response of Z. mellis 6-7431 to high-glucose stress was characterized. Its morphology and reproduction pattern under high-glucose stress were observed using scanning electron microscopy(SEM). The concentrations of metabolites such as glycerol, trehalose and malic acid were also determined. Results showed that Z. mellis 6-7431 exhibited a normal budding reproduction and the concentrations of these metabolites were elevated in the glucose concentration range of 300-500 g/L relative to a 100 g/L concentration. When glucose concentration increased from 550 to 700 g/L, Z. mellis 6-7431 started to form spores gradually, and the concentrations of these metabolites varied. At a high glucose concentration of 750 g/L,strain 6-7431 was dehydrated and its morphology became wizened. All the metabolic activities of this strain were inhibited,and the alcoholic concentration was only 0.6% in the fermented broth. Thesefindings facilitated our understanding of the physiological response to high-glucose exposure and glucose-tolerant mechanism of Z. mellis 6-7431.

关 键 词：蜂蜜接合酵母 高糖胁迫 繁殖方式 应激代谢产物 

分 类 号：Q93[生物学—微生物学]