高产脂肪酶菌株的筛选鉴定及产酶条件优化  

Screening and identification of high yield lipase strains and optimization of enzyme production conditions

作　　者：刘延波[1,2] 邢星月 赵志军[1,2] 刘宁[1,2] 潘春梅[1,2] 孙西玉[1,2,3] LIU Yanbo;XING Xingyue;ZHAO Zhijun;LIU Ning;PAN Chunmei;SUN Xiyu(College of Food and Biological Engineering (Liquor College),Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Henan Liquor Style Engineering Technology Research Center,Henan University of Animal Husbandry and Economy, Zhengzhou 450046,China;ZhangGongLaoJiu Wine Co.,Ltd.,Ningling 476733,China)

机构地区：[1]河南牧业经济学院食品与生物工程学院(酒业学院),河南郑州450046 [2]河南牧业经济学院河南省白酒风格工程技术研究中心,河南郑州450046 [3]河南张弓老酒酒业有限公司,河南宁陵476733

出　　处：《中国酿造》2019年第7期54-59,共6页China Brewing

基　　金：河南牧业经济学院博士科研启动资金项目;河南牧业经济学院青年科研创新基金项目[XKYCXJJ2017015];河南牧业经济学院重点学科项目;河南省高等学校重点科研项目[17A180005];河南省重大科技专项[181100211400]

摘　　要：采用罗丹明B平板法和脂肪酶活力测定从张弓老酒大曲中分离筛选出一株高产脂肪酶菌株E-11,经形态学、生理生化试验和16SrDNA序列分析,鉴定该菌株为枯草芽孢杆菌(Bacillus subtilis)。通过单因素和响应面试验设计对该菌株的产酶条件进行优化,结果表明,最佳产酶条件为葡萄糖3%、蛋白胨4%、初始pH值为8.0。优化后的脂肪酶活力达15.09U/mL,是优化前产酶能力的1.2倍。A high yield lipase strain E-11 was isolated and screened from Daqu of Zhanggonglaojiu by rhodamine B plate method and lipase activity determination. After morphological,physiological and biochemical tests and 16S rDNA sequences analysis,the strain was identified as Bacillus subtilis. The optimum conditions of enzyme production were optimized by single factor tests and response surface methodology and were determined as follows: glucose 3%,peptone 4%,and initial pH 8.0. Under the optimal conditions,the lipase activity reached 15.09 U/ml,which was 1.2 times that of before optimization.

关 键 词：大曲 脂肪酶 分离鉴定 响应面试验 发酵条件优化 

分 类 号：Q936[生物学—微生物学]