天然植物精油对枯草芽孢杆菌的抑制作用  

Antibacterial Effect of Plant Essential Oil on Bacillus subtilis

作　　者：王叶青[1] 丁慧璞 徐旭 程丹 王志江 刘利萍[1] Wang Yeqing;Ding Huipu;Xu Xu;Cheng Dan;Wang Zhijiang;Liu Liping(College of Biological & Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,Zhejiang;Ningbo Dayang Industry and Trade Co.,Ltd,Ningbo 315000,Zhejiang;Department of Pharmaceutical Engineering,Zhejiang Pharmaceutical College,Ningbo 315100,Zhejiang)

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100 [2]宁波大央工贸有限公司,浙江宁波315000 [3]浙江医药高等专科学校制药工程学院,浙江宁波315100

出　　处：《中国食品学报》2019年第7期72-78,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省“生物工程”重中之重学科学生创新计划项目(CX2016005);浙江省一流学科“生物工程”开放基金项目

摘　　要：为了研究天然植物精油对枯草芽孢杆菌的抑菌作用,采用倍比稀释法对8种植物精油(菊花、冬青、金盏花、八角茴香、香蜂草、佛手柑、茉莉和野番茄精油)的抑菌活性进行筛选。采用Box-Behnken响应面试验设计研究精油间可能存在的协同增效作用。绘制复配精油对枯草芽孢杆菌的生长曲线,以判断其抑菌特征。结果表明,当精油含量在1μL/mL内,除香蜂草精油、野番茄精油外,其余6种精油对枯草芽孢杆菌都有抑制活性。八角茴香精油(MIC=0.5μL/mL)的抑菌活性比常见防腐剂(尼泊金酯)还强。当冬青精油0.61μL/mL+菊花精油0.44μL/mL+金盏花精油0.24μL/mL+八角茴香精油0.17μL/mL,冬青精油0.5μL/mL、八角茴香精油0.13μL/mL时,菊花精油和金盏花精油存在协同增效作用。由枯草芽孢杆菌的生长曲线可知,复配精油主要抑制了枯草芽孢杆菌对数生长期的分裂和繁殖,使得活菌数明显下降,无法达到正常的生长高峰。In order to study the antibacterial effect of plant essential oil on Bacillus subtilis, the bacteriostatic activities of eight plant essential oils (chrysanthemum, holly, marigold, anise, vanilla, bergamot, jasmine and wild tomato essential oil) were preliminarily screened by double dilution method. Box-Behnken response surface design was used to study possible synergistic effects between essential oils. The growth curve of compound essential oil against Bacillus subtilis was drawn to judge its bacteriostatic characteristics. The results showed that the other six essential oils excepting vanilla and wild tomato oil had inhibitory activities against Bacillus subtilis within 1 μL/mL. The antimicrobial activity of anise essential oil (MIC=0.5 μL/mL) was stronger than that of common preservatives (paraben). When the essential oils of holly and anise were 0.13 μL/mL and 0.5 μL/mL, respectively, chrysanthemum essential oil and Calendula essential oil had synergistic effect. According to the compound essential oil of holly essential oil 0.61 μL/mL+ chrysanthemum essential oil 0.44 μL/mL+ calendula essential oil 0.24 μL/mL+ anise essential oil 0.17 uL/mL, the transmittance of bacterial liquid was 91.05%+1.81%. According to the growth curve of Bacillus subtilis, the mixed essential oil mainly inhibited the division and reproduction of the logarithmic growth phase of Bacillus subtilis. And the number of living bacteria decreased obviously, which could not reach the normal growth peak.

关 键 词：植物精油 抑菌活性 枯草芽孢杆菌 复配 

分 类 号：TS202.3[轻工技术与工程—食品科学]