双酶分步酶解提取羊骨中可溶性钙  

Extraction of soluble calcium from sheep bone by two-step enzymolysis

作　　者：王雪琦 张珍[1] 徐红艳 刘倩[1] 李晓叶 郝旭东 赵文宝 周芸 WANG Xueqi;ZHANG Zhen;XU Hongyan;LIU Qian;LI Xiaoye;HAO Xudong;ZHAO Wenbao;ZHOU Yun(College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;Qingyang Food Inspection and Testing Center, Qingyang 745000, China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]庆阳市食品检验检测中心,甘肃庆阳745000

出　　处：《食品与发酵工业》2019年第15期143-149,共7页Food and Fermentation Industries

基　　金：中国-马来西亚清真食品及相关产业国际创新合作(1504WKCA094);中国-马来西亚在清真食品生物科技、检测及标准体系的合作研究(17YF1WA166);科技部援助项目(KY201501005);甘肃省财政厅高校基本业务项目(1011JKCA179);甘肃省农牧厅生物技术专项(GNSW-2013-22);甘肃省科技计划资助(17YF1WA166)

摘　　要：为提高羊骨的高值利用,研究了木瓜蛋白酶和胃蛋白酶分步酶解提取羊骨中可溶性钙的最佳工艺条件。以羊骨粉为原料,游离钙含量为指标,通过单因素试验和响应面优化2种酶的酶解工艺,并在不同加入顺序和时间组合下进行分步水解,提取羊骨中可溶性钙。结果表明,木瓜蛋白酶和胃蛋白酶的最佳酶解条件分别为酶解温度55、40℃,酶解时间均为4h,酶解pH6.0、2.5,加酶量5000、5300U/g,所测实际游离钙含量是79.02和1008.68mg/100g;在双酶各自最优酶解条件下,按先木瓜蛋白酶后胃蛋白酶的加入顺序,分别酶解3h,游离钙含量达到了1839.14mg/100g,极显著高于单酶和其他组合(P<0.01)。The optimum condition for extracting soluble calcium from sheep bone by papain and pepsin was studied. Using free calcium content as an index, the enzymatic hydrolysis processes of papain and pepsin were optimized by single factor test and response surface analysis, and the two step enzymolysis was carried out under different addition sequences and time combinations of the two enzymes. The results showed that the optimal extraction conditions for papain and pepsin were at 55 ℃, pH 6.0 and at 40 ℃, pH2.5, hydrolyzed for 4 h with the additions of 5 000 U/g and 5300 U/g, respectively. Under these conditions, the free calcium contents extracted were 79.02 mg/100 g and1 008.68 mg/100g, respectively. Based on the optimal hydrolysis conditions of papain and pepsin, the optimum condition for the two-step enzymolysis was to add papain first followed by pepsin and hydrolyzed for3 h each. Under this condition, the free calcium content reached1 839.14 mg/100 g, which was significantly higher than those of single enzyme extraction and other combinations ( P <0.01). In conclusion, this study intended to lay a foundation for high value utilization of sheep bones and development of related products.

关 键 词：羊骨粉 木瓜蛋白酶 胃蛋白酶 酶解 可溶性钙 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]