Dynamic Variation of RAS on Silicotic Fibrosis Pathogenesis in Rats  

作　　者：Bo-nan ZHANG Xin ZHANG Hong XU Xue-min GAO Gui-zhen ZHANG Hui ZHANG Fang YANG 

机构地区：[1]School of Public Health,Tangshan Key Laboratory for Clinical and Basic Research on Chronic Diseases,School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063210,China [2]Hebei Key Laboratory for Organ Fibrosis,Medical Research Center,Tangshan Key Laboratory for Clinical and Basic Research on Chronic Diseases,School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063210,China [3]Hebei Key Laboratory for Chronic Diseases,Tangshan Key Laboratory for Clinical and Basic Research on Chronic Diseases,School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063210,China [4]Basic Medical College,Hebei Medical University,Shijiazhuang 050017,China

出　　处：《Current Medical Science》2019年第4期551-559,共9页当代医学科学（英文）

基　　金：This study was supported by grants from the National Natural Science Foundation of China (No.81472953);Natural Science Foundation of Hebei Province (No.H2016209170);Graduate Student Innovation Fund of Hebei Province (No.2016196);Graduate Student Innovation Fund of North China University of Science and Technology (No.2016B10);Undergraduate Innovative Project of North China University of Science and Technology (No.X2017354).

摘　　要：The dynamic variation of renin-angiotensin system (RAS) in silicosis remains unclear.Seventy Wistar rats were divided into 7 groups including control group,silicosis groups (inhaling SiO2 for 2,4,8,16 and 24 weeks,respectively) and Captopril (Cap) group.Rat lung primary fibroblasts were divided into control group,SiO2-stimulated group (0,0.5,1,3,6,12,24 and 48 h) and Cap group.The silicotic nodules were formed and collagens were deposited gradually in silicosis group observed by haematoxylin and eosin (HE) staining and Van Gieson (VG) staining.Cap relieved the lung fibrosis and collagen deposition.Immunohistochemistry indicated the positive expression of α-smooth muscle actin α-SMA) was increased gradually in silicotic rat lung tissue.Western blotting revealed the expression of collagen type Ⅰ(Col Ⅰ) and α-SMA was up-regulated in silicotic rat lung tissue and fibroblasts stimulated by SiCh.Cap decreased the expression of Col Ⅰ and α-SMA in silicotic rat lung tissue and fibroblasts stimulated by SiCh.Western blotting also demonstrated the expression of angiotensin-converting enzyme (ACE) and angiotensin Ⅱ type 1 receptor (ATI) was increased,and the expression of ACE2 and Mas was decreased gradually in silicotic rat lung tissue and fibroblasts stimulated by SiCh.ELISA showed the serum levels of ACE and angiotensin Ⅱ(Ang Ⅱ) were also increased and ACE2 and Ang (1 -7) were decreased in the silicosis group.Treatment with Cap decreased the expression levels of ACE,Ang Ⅱ and ATI,and increased the expression levels of ACE2,Ang (1-7) and Mas.These findings suggested that an imbalance between ACE-Ang Ⅱ-AT1 axis and ACE2-Ang (l-7)-Mas axis may participate in the development of silicosis.

关 键 词：SILICOSIS FIBROBLASTS SiCh RENIN-ANGIOTENSIN system α-smooth muscle ACTIN 

分 类 号：R[医药卫生]