茂原链霉菌谷氨酰胺转氨酶合成与菌体形态分化的关系  

Relationship between TGase Production and Morphological Differentiation of Streptomyces mobaraensis

作　　者：薛慧 付玲 李洪波[3] 王淑梅 刘宁[2] 张莉丽[2] XUE Hui;FU Ling;LI Hongbo;WANG Shumei;LIU Ning;ZHANG Lili(Jiamusi College,Heilongjiang University of Traditional Chinese Medicine,Jiamusi 154007,China;College of Food Science,Northeast Agricultural University,Harbin 150030,China;College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;College of Food Engineering,Harbin University,Harbin 150086,China)

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [2]东北农业大学食品学院,黑龙江哈尔滨150030 [3]天津科技大学食品工程与生物技术学院,天津300457 [4]哈尔滨学院食品工程学院,黑龙江哈尔滨150086

出　　处：《食品科学》2019年第20期130-135,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目（NSFC31601496）;中国博士后基金面上项目（2014M560244）;中国博士后基金国际交流项目（20150082）;黑龙江省博士后基金项目（LBH-Z13042）;黑龙江省高校青年创新人才项目（UNPYSCT-2016148）;哈尔滨市科技局青年后备人才项目(2016RAQXJ143)

摘　　要：目的:研究茂原链霉菌(Streptomyces mobaraensis)合成谷氨酰胺转氨酶(transglutaminase,TGase)与菌体分化之间的关系,以及TGase的生理功能.方法:以S.mobaraensis为出发菌株,通过向培养基中添加乙二胺四乙酸(ethylenediaminetetraacetic acid,EDTA)抑制TGase激活关键蛋白酶的活性从而调控成熟TGase的合成,同时利用激光共聚焦显微镜和扫描电镜观察发酵过程中菌体的活力和形态变化.结果:对照组随着TGase产量的升高,菌体生存活力呈先上升后下降的趋势,并且菌体表现出复杂的形态变化;实验组自加入EDTA后TGase产量不再升高,菌体生存活力也逐渐减弱,菌体形态分化滞后.结论:TGase的合成会影响茂原链霉菌生存活力及其形态分化.The purposes of the present study were to clarify the relationship between the morphological differentiation and transglutaminase (TGase) production Streptomyces mobaraensis, and to explore the physiological role of TGase in S. mobaraensis development. EDTA was added into the fermentation medium to inhibit the activities of the key proteases for TGase biosynthesis, and then the viability and morphological differentiation of the pellets were investigated using a confocal laser scanning microscope (CLSM) and a scanning electron microscope (SEM). The viability of S. mobaraensis pellets in the control group increased initially and decreased afterwards as the TGase activity increased, and the mycelia showed complex morphological changes. In the experimental group, a significant inhibition effect of EDTA was observed on TGase production, and the viability of S. mobaraensis pellets was decreased upon the addition of EDTA. In addition, mycelial morphological differentiation was delayed. These results suggest that TGase biosynthesis affects the viability of pellets and mycelial morphological differentiation of S. mobaraensis.

关 键 词：谷氨酰胺转氨酶 茂原链霉菌 菌体活力 形态分化 生理功能 

分 类 号：TS201.3[轻工技术与工程—食品科学]