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作 者:赵彩艳 朱肖亭 王二耀[1] 徐照学[1] 施巧婷[1] ZHAO Cai-yan;ZHU Xiao-ting;WANG Er-yao;XU Zhao-xue;SHI Qiao-ting(Henan Provincial Key Laboratory of Livestock and Poultry Breeding and Nutrition Regulation,Institute of Animal Science and Veterinary Medicine,Henan Academy of Agricultural Sciences,Zhengzhou 450000,China)
机构地区:[1]河南省农业科学院畜牧兽医研究所河南省畜禽繁育和营养调控重点实验室
出 处:《畜牧与饲料科学》2019年第7期31-34,共4页Animal Husbandry and Feed Science
基 金:国家肉牛牦牛产业技术体系(CARS-38);河南省肉牛产业技术体系资助
摘 要:采用单因素实验设计,对酵母菌发酵,以玉米粉和麸皮为主要培养基原料的固体发酵培养基配方进行了筛选。以接种量、料水比例、发酵温度、发酵周期等因素作为研究对象,确定了最佳发酵条件。结果表明:筛选出的培养基配方为:玉米粉75%,麸皮21%,NH4NO3 2%,KH2PO4 1%,MgSO4 1%。适宜的发酵条件为:接种量12%,料水比例1∶1.5,发酵温度30℃,发酵周期66 h,该条件下发酵终产物粗蛋白含量最高,为20.56%。The solid-state fermentation medium formula mainly composed of corn powder and bran with yeast as fermentation starter was screened and optimized by single factor experiment design method. The inoculation quantity, ratio of material to water, fermentation temperature, fermentation time and other factors were used as the evaluation indicators, and the best fermentation conditions were determined. The results showed that the screened best culture medium formula was as follows: 75% of corn powder, 21% of bran, 2% of NH4NO3, 1% of KH2PO4, 1% of MgSO4. Furthermore, the optimum fermentation conditions were as follows: the inoculation quantity was 12%, the ratio of material to water was 1∶1.5, the fermentation temperature was 30 ℃, and the fermentation duration was 66 h. The highest crude protein content (20.56%) of the final fermentation product was obtained under this condition.
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