葛仙米藻蓝蛋白和藻红蛋白的体外抗炎和抗紫外活性比较研究  

Comparative Studies on the Anti-Inflammatory and Uvioresistant Activity of Phycocyanin and Phycoerythrin in Nostoc sphaeroides Kutzing in Vitro

作　　者：余佳 张瑞华 王生 许文琦[2,3] 王玉兰 YU Jia;ZHANG Rui-hua;WANG Sheng;XU Wen-qi;WANG Yu-lan(Hunan Micro Algae Biological Engineering Technology Research Center,Hunan Yandi Bioengineering Co.,Ltd.,Zhuzhou 412007,China;China State Institute of Pharmaceutical Industry,Shanghai 200040,China;State Key Laboratory of New Drug&Pharmaceutial Process,Shanghai Institute of Pharmaceutial Industry,Shanghai 200437,China)

机构地区：[1]湖南炎帝生物工程有限公司,湖南省微藻生物工程技术研究中心,湖南株洲412007 [2]中国医药工业研究总院,上海200040 [3]上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200437

出　　处：《食品工业科技》2019年第17期299-303,共5页Science and Technology of Food Industry

摘　　要：目的:对葛仙米藻蓝蛋白和藻红蛋白的抗炎和抗紫外活性进行体外比较研究。方法:通过Lipopolysaccharide(LPS)诱导RAW264.7细胞分泌NO和TNF-α,比较葛仙米中藻蓝蛋白和藻红蛋白的抗炎活性;通过Ultraviolet radiation B(UVB)辐照诱导NIH-3T3成纤维细胞损伤,考察藻蓝蛋白和藻红蛋白的抗紫外损伤活性。结果:体外抗炎结果显示LPS能极显著诱导RAW264.7细胞分泌NO和TNF-α(P<0.01),藻蓝蛋白和藻红蛋白均能极显著抑制LPS诱导RAW264.7分泌的NO和TNF-α的含量水平(P<0.01),其中藻红蛋白抑制NO分泌能力高于藻蓝蛋白,藻蓝蛋白对TNF-α的分泌抑制作用高于藻红蛋白。体外抗紫外损伤结果显示UVB 4000 mJ/cm2可以成功诱导NIH-3T3细胞的紫外损伤,但是藻蓝蛋白和藻红蛋白对NIH-3T3细胞的紫外损伤并无明显保护作用。结论:葛仙米中藻蓝蛋白和藻红蛋白具有很好的体外抗炎活性,但两者对UVB诱导的细胞损伤并无明显保护作用。Objective:Comparative studies on the anti-inflammatory and uvioresistant activity of phycocyanin and phycoerythrin in Nostoc sphaeroides Kutzing were developed in vitro.Methods:Raw264.7 cells induced by LPS were used to evaluate the antiinflammatory activity through detecting NO and TNF-αsecretion.NIH-3T3 cell damaged by UVB was used to evaluate the uvioresistant activity.Results:Raw264.7 cells induced by LPS secreted NO and TNF-αextremely significantly(P<0.01),and phycocyanin and phycoerythrin extremely significantly reduced the level of NO and TNF-αsecretion(P<0.01).Phycoerythrin was more effective than phycocyanin on the ability of NO secretion inhibition.And phycocyanin was more effective than phycoerythrin on the ability of TNF-αsecretion inhibition.UVB 4000 mJ/cm2 inhibited the viability of NIH-3T3 cells successfully,but phycocyanin and phycoerythrin showed no protection efficacy on UVB induced cells damage.Conclusion:Phycocyanin and phycoerythrin shows significant anti-inflammatory activity,but there is no protection efficacy on UVB induced cells damage.

关 键 词：葛仙米 藻蓝蛋白 藻红蛋白 抗炎 抗紫外 

分 类 号：TS201.4[轻工技术与工程—食品科学]