基质匹配溶剂校准-GC-MS/MS法测定卷烟主流烟气B[a]P  

Determination of benzo[a]pyrene in mainstream cigarette smoke using matrix matched solvent calibration-gas chromatography/tandem mass spectrometry method

作　　者：廖惠云[1] 王瑞[1] 张华[1] 吴洋[1] 曹毅[1] 张媛[1] 王晨辉[1] LIAO Huiyun;WANG Rui;ZHANG Hua;WU Yang;CAO Yi;ZHANG Yuan;WANG Chenhui(China Tobacco Jiangsu Industrial Co.,Ltd.,Nanjing 210019,China)

机构地区：[1]江苏中烟工业有限责任公司

出　　处：《烟草科技》2019年第9期55-63,共9页Tobacco Science & Technology

基　　金：江苏中烟工业有限责任公司科技项目“细支卷烟用爆珠对逐口烟气中主要香味物质释放量影响研究”(201703)、“细支卷烟中香精料液代表性组分释放转移分析及应用研究”(201704)

摘　　要：为建立基质匹配溶剂校准-GC-MS/MS法快速测定卷烟主流烟气中B[a]P的方法,卷烟烟气经捕集后,采用振荡提取方式,萃取液直接进行色谱分析。考察了基质匹配溶剂的制备过程,比较了色谱进样方式、质谱参数等条件对目标物出峰情况的影响,优选了样品前处理步骤及样品提取方式。结果表明:①在优化条件下,目标物在1.04~20.8 ng/mL浓度范围内线性良好(R2为0.999 6),加标回收率在94.62%~104.81%之间,日内和日间相对标准偏差(RSDs)分别介于3.13%~3.80%和5.41%~6.94%之间,检出限为0.14 ng/支,定量限为0.46 ng/支,低于国标方法报道值。②18个不同盒标焦油量的细支和常规卷烟分析结果显示,本方法测定结果与现行国标方法基本一致。该方法具有操作简便、耗费溶剂少、灵敏度高等特点,适合于卷烟主流烟气B[a]P释放量的快速测定。A novel method to quantify trace benzo[a]pyrene(B[a]P)in mainstream cigarette smoke by matrix matched solvent calibration-gas chromatography/tandem mass spectrometry(MMSC-GC-MS/MS)was developed.The chromatography was conducted by injecting the extract directly after extracting Cambridge filters with oscillating extraction method.The preparation process of matrix matched solvent,injection pattern and MS parameters were investigated,and sample preparation steps and target’s extraction pattern were optimized.The results showed that:1)Under the optimized conditions,the calibration curve was linear(R2=0.999 6)in the target concentration range of 1.04-20.8 ng/mL with the limits of detection and quantitation at 0.14 and 0.46 ng/cig.respectively,which were lower than the reported values of the current Chinese National Standard method.The spiked recoveries of different samples ranged from 94.62%to 104.81%with intra-and inter-day relative standard deviations(RSDs)at 3.13%-3.80%and 5.41%-6.94%,respectively.2)No significant deviation was observed between the results of B[a]P in mainstream smoke of 18 slim and conventional cigarettes with different labeled tar levels determined by the proposed MMSC-GC-MS/MS method and the Chinese National Standard method.This method was suitable for the rapid determination of B[a]P in mainstream cigarette smoke with the advantages of convenience,lower organic solvent consumption and higher sensitivity.

关 键 词：卷烟 主流烟气 气相色谱-串联质谱 基质匹配溶剂 苯并[a]芘(B[a]P) 

分 类 号：TS411.2[农业科学—烟草工业]