酶解预处理对水酶法提取牡丹籽油提油率的影响  

Effect of Enzymolysis Pretreatment on Peony Seeds Oil Extraction Rate by Aqueous Enzymatic Method

作　　者：王青[1] 王晓东 程安玮[1] 徐同成[1] 王新坤[1] 孙金月[1] WANG Qing;WANG Xiao-dong;CHENG An-wei;XU Tong-cheng;WANG Xin-kun;SUN Jin-yue(Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-products Processing Technology of Shandong Province/Institute of Agro-food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]农业农村部新食品资源加工重点实验室/山东省农产品精深加工技术重点实验室/山东省农业科学院农产品研究所

出　　处：《中国食物与营养》2019年第10期26-29,共4页Food and Nutrition in China

基　　金：国家重点研发计划子课题“多酚油料功能脂质高效制备与评价技术研究”(项目编号:2018YFD0401104-4);山东省泰山学者特聘专家人才项目(项目编号:tshw20120747);山东省自然科学基金项目(项目编号:ZR2019MC072)

摘　　要：为提高水酶法提取牡丹籽油的出油率,研究了果胶酶和纤维素酶复合酶预处理对提油量的影响,结果表明:果胶酶与纤维素酶比例为2∶1时,提油量最高;通过单因素及响应曲面试验设计优化得到复合酶酶解预处理牡丹籽提油的最佳工艺条件为:pH 4.3、复合酶添加量2.5%、酶解温度47℃、酶解时间2.3h。该工艺参数下进行前处理,再用碱性蛋白酶酶解,每10g牡丹籽的提油量为1.345 8g,换算成提油率约为85%。The effect of pretreatment with complex enzymes of pectinase and cellulase on peony seeds oil extraction rate was studied.The results showed that the peony seeds oil extraction rate was the highest when the ratio of the amount of pectinase to cellulase was 2∶1.The optimal conditions for pretreatment of peony seeds for oil extra ction by single-factor tests and response surface method were pH 4.3,enzyme dosage 2.5%,enzymolysis temperature 47℃,and enzymolysis time 2.3 h.Under the above pretreatment conditions combined with alkaline protease enzymolysis,the peony seeds oil extraction rate was up to 1.345 8g per 10g,and the oil extraction rate was about 85%.

关 键 词：牡丹籽油 水酶法 复合酶 提油率 

分 类 号：TS2[轻工技术与工程—食品科学与工程]