纤维素酶和木聚糖酶协同降解葡萄皮渣条件优化  

Optimization of conditions for cellulase and xylanase to co-degrade grape pomace

作　　者：索江华[1] 唐桂芬[1] 连艳鲜[1] SUO Jianghua;TANG Guifen;LIAN Yanxian(Dpartment of Food and Biological Engineering,Henan University of Animal Husbandy and Economy,Zhengzhou,Henan Province 450046,China)

机构地区：[1]河南牧业经济学院食品与生物工程学院

出　　处：《中国饲料》2019年第21期113-117,共5页China Feed

基　　金：河南省科技攻关项目(182102110125;182102110021;182102110188)

摘　　要：为提高纤维素酶和木聚糖酶协同作用对葡萄皮渣的降解效果,首先通过单因素试验对纤维素酶和木聚糖酶的浓度配比、酶反应温度(℃)、酶反应时间(h)、pH等条件进行研究,得出单因素降解的最佳条件,再利用响应面法进行设计和分析,得到最佳组合工艺:纤维素酶与木聚糖酶的比例为5:4,温度60℃,pH 4.0,反应12 h,处理1 g葡萄皮渣,葡萄皮渣中还原糖含量提高了44.4%,粗纤维下降了28.34%。In order to improve the degradation effect of cellulase and xylanase on grape pomace.First,conditions such as concentration ratio of cellulase and xylanase,enzyme reaction temperature(℃),pH,enzyme reaction time(h)were explored by single factor test,and response surface analysis were adopted to further investigate mutual interaction between variables.The results showed that the optimal conditions were as follows:The ratio of cellulase to xylenase was 5:4,the temperature was 60℃,and the reaction time was 12 hours at pH 4.0,1 g grape peel residue was treated.The reducing sugar of the determination of grape peel residue was increased by 44.4%,and the crude fiber was decreased by 28.34%.

关 键 词：葡萄皮渣 纤维素酶 木聚糖酶 响应面 

分 类 号：S816.5[农业科学—饲料科学]