混合型苦瓜保健酒的发酵工艺优化及其抗氧化活性研究  

Fermentation technology optimization and antioxidant activity of mixed Momordica charantia health wine

作　　者：那广宁[1] 徐铭悦 郑诗绮 李云跃 汪琢[1] NA Guangning;XU Mingyue;ZHENG Shiqi;LI Yunyue;WANG Zhuo(College of Life Engineering,Shenyang Institute of Technology,Fushun 113122,China)

机构地区：[1]沈阳工学院生命工程学院

出　　处：《中国酿造》2019年第11期199-204,共6页China Brewing

基　　金：沈阳市科技计划项目(18-013-0-07)

摘　　要：以苦瓜汁与苹果菠萝混合果汁为原料,选用酿酒酵母(Saccharomyces cerevisiae)及植物乳杆菌(Lactobacillus plantarum)作为发酵菌种定向发酵苦瓜保健酒。以酒精度、感官评价及苦瓜皂苷产率为评价指标,采用Box-Behnken中心组合试验优化发酵条件,同时以维生素C作为阳性对照,采用清除羟自由基和Fe3+还原能力分析苦瓜保健酒的抗氧化活性。结果表明,苦瓜保健酒的最佳发酵工艺条件为苹果菠萝混合果汁∶苦瓜汁=1.5∶1、发酵时间10 d、酵母添加量为1 mL/L、乳酸菌添加量6 mL/L、发酵温度26℃的条件下可生产出质量最佳的苦瓜保健酒。此条件下成品果酒的品质最佳,皂苷含量达到2.78%,酒精度为11.2%vol。抗氧化试验结果表明,苦瓜保健酒有一定的抗氧化活性,0.1 mg/mL的苦瓜保健酒对羟自由基的清除率达到25%以上,对Fe3+也有一定还原能力,且其抗氧化化性与皂苷质量浓度呈正相关。Using Momordica charantia juice,mixed apple and pineapple fruit juice as raw materials,M.charantia health wine was fermented with Saccharomyces cerevisiae and Lactobacillus plantarum as fermentation strains.Using alcohol content,sensory evaluation and M.charantia saponin yield as evaluation indexes,the fermentation conditions were optimized by Box-Behnken central combination tests.Using vitamin C as positive control,the antioxidant activity of M.charantia health wine was analyzed by scavenging hydroxyl radicals and reducing Fe3+capacity.The results showed that the optimum fermentation conditions of M.charantia health wine were mixed apple and pineapple juice∶M.charantia juice 1.5∶1,fermentation time 10 d,yeast addition 1 ml/L,lactic acid bacteria addition 6 ml/L,and fermentation temperature 26℃.Under these conditions,the quality of fruit wine was the optimal with saponin content 2.78%and alcohol content 11.2%vol.The results of antioxidant tests showed that M.charantia health wine had certain antioxidant activity.The scavenging rate of hydroxyl radicals in M.charantia health wine 0.1 mg/ml was more than 25%,and it also had certain reduction ability to Fe3+and its antioxidant activity was positively correlated with the content of saponins.

关 键 词：苦瓜 苦瓜皂苷 保健酒 发酵 抗氧化 

分 类 号：Q815[生物学—生物工程]