Profiling of lysine-acetylated proteins in human urine  

作　　者：Weiwei Qin Ting Wang He Huang Youhe Gao 

机构地区：[1]Department of Biochemistry and Molecular Biology,Gene Engineering Drug and Biotechnology Beijing Key Laboratory,Beijing Normal University,Beijing 100875,China [2]Department of Anesthesiology,Qingdao Municipal Hospital,Qingdao 266071,China

出　　处：《Science China(Life Sciences)》2019年第11期1514-1520,共7页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFC0910202,2016YFC1306300);the Beijing Natural Science Foundation(7172076);the Beijing cooperative construction project(110651103);the Beijing Normal University(11100704);the Peking Union Medical College Hospital(2016-2.27)

摘　　要：A biomarker is a measurable indicator associated with changes in physiological state or disease.In contrast to the blood which is under homeostatic controls,urine reflects changes in the body earlier and more sensitively,and is therefore a better biomarker source.Lysine acetylation is an abundant and highly regulated post-translational modification.It plays a pivotal role in modulating diverse biological processes and is associated with various important diseases.Enrichment or visualization of proteins with specific post-translational modifications provides a method for sampling the urinary proteome and reducing sample complexity.In this study,we used anti-acetyllysine antibody-based immunoaffinity enrichment combined with high-resolution mass spectrometry to profile lysine-acetylated proteins in normal human urine.A total of 629 acetylation sites on 315 proteins were identified,including some very low-abundance proteins.This is the first proteome-wide characterization of lysine acetylation proteins in normal human urine.Our dataset provides a useful resource for the further discovery of lysine-acetylated proteins as biomarkers in urine.A biomarker is a measurable indicator associated with changes in physiological state or disease. In contrast to the blood which is under homeostatic controls, urine reflects changes in the body earlier and more sensitively, and is therefore a better biomarker source. Lysine acetylation is an abundant and highly regulated post-translational modification. It plays a pivotal role in modulating diverse biological processes and is associated with various important diseases. Enrichment or visualization of proteins with specific post-translational modifications provides a method for sampling the urinary proteome and reducing sample complexity. In this study, we used anti-acetyllysine antibody-based immunoaffinity enrichment combined with high-resolution mass spectrometry to profile lysine-acetylated proteins in normal human urine. A total of 629 acetylation sites on 315 proteins were identified, including some very low-abundance proteins. This is the first proteome-wide characterization of lysine acetylation proteins in normal human urine. Our dataset provides a useful resource for the further discovery of lysine-acetylated proteins as biomarkers in urine.

关 键 词：URINE POST-TRANSLATIONAL modification LYSINE ACETYLATION 

分 类 号：R73[医药卫生—肿瘤]