鲣鱼黄嘌呤氧化酶抑制肽酶法制备工艺优化  

Optimization of enzymatic hydrolysis process for preparing xanthine oxidase inhibitory peptides from skipjack tuna

作　　者：邹琳 杭妙佳 李阳[1] 杜鹃 冯凤琴[1] ZOU Lin;HANG Miaojia;LI Yang;DU Juan;FENG Fengqin(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;Hangzhou Kangyuan Food Science&Technology Co.,Ltd.,Hangzhou 310003,China)

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310058 [2]杭州康源食品科技有限公司,杭州310003

出　　处：《浙江大学学报（农业与生命科学版）》2019年第5期550-562,共13页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江大学科技合作项目“水产动物源功能蛋白肽产品研究开发”(K横20182914);浙江大学实验技术研究项目(SJS201708)

摘　　要：以鲣鱼为原料,利用酶法制备鲣鱼黄嘌呤氧化酶(xanthine oxidase,XOD)抑制肽。在单因素试验的基础上,采用Box-Behnken响应面分析法,以水解度、氮回收率、XOD抑制活性、肌肽及鹅肌肽含量为响应值,对酶解工艺中蛋白酶种类、加酶量、酶解pH、温度和时间等影响因素进行优化,得到酶解的最优工艺:采用中性蛋白酶,加酶量为489.86 U/g,酶解pH为7.08,酶解温度为49.5℃,酶解时间为5 h。在此条件下,鲣鱼酶解产物的水解度为22.38%,氮回收率为83.31%,XOD抑制活性达到62.26%,肌肽和鹅肌肽含量分别为0.05%和2.45%(以干基计),与回归模型理论预测值基本一致。由本法所得的鲣鱼XOD抑制肽是以分子质量低于1000 Da的寡肽为主。皮尔逊相关性分析表明,XOD抑制活性与肌肽或鹅肌肽含量无相关性,推测在酶解过程中产生了其他具有XOD抑制活性的小分子肽。Skipjack tuna was enzymatically hydrolyzed to prepare xanthine oxidase(XOD)inhibitory peptides.The enzymatic hydrolysis process was optimized by investigating the effects of protease types and enzymatic dosages,p H,temperature,time and their interactions on degree of hydrolysis,nitrogen recovery,XOD inhibition activity and the contents of carnosine and anserine using one-factor-at-a-time method and response surface methodology.The optimal process parameters were as follows:the application of neutrase with the dosage of 489.86 U/g,the pH of 7.08,the temperature of 49.5℃,and the time of 5 h.Under the optimal conditions,the degree of hydrolysis was 22.38%;nitrogen recovery was 83.81%;XOD inhibition activity was 62.26%;and the contents of carnosine and anserine were 0.05%and 2.45%(on a dried basis),respectively,which were all in good agreement with the predicted values.The XOD inhibitory peptides obtained were mainly composed of a fraction with molecular mass of less than 1000 Da.The Pearson’s correlation analysis showed that the XOD inhibition activity of dorsal and ventral muscle in skipjack tuna was not associated with the contents of carnosine and anserine,and it may be due to the production of other XOD inhibitory peptides during the enzymatic hydrolysis.

关 键 词：鲣鱼 黄嘌呤氧化酶抑制肽 酶解工艺 肌肽 鹅肌肽 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]