高抗氧化性小分子阿胶的研究  

Preparation of a high-antioxidant hydrolysate from Colla Corii Asini

作　　者：杜博玮 徐晓冰 郭尚伟 黄雅钦[1] DU BoWei;XU XiaoBing;GUO ShangWei;HUANG YaQin(College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]北京化工大学材料科学与工程学院

出　　处：《北京化工大学学报（自然科学版）》2019年第6期15-20,共6页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：中央高校基本科研业务费(PYBZ1805)

摘　　要：用碱性蛋白酶对阿胶进行酶解,并采用超滤技术分离制备了平均分子量小于1 kDa的小分子阿胶(CCAH-Ⅱ)。通过多种抗氧化评价方法分析发现制备的CCAH-Ⅱ具有较高的抗氧化活性,质量浓度2 mg/mL的CCAH-Ⅱ对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基和羟基自由基的清除率分别可以达到(53.79±1.18)%、(70.53±0.65)%、(80.00±0.45)%。通过氨基酸组成和圆二色谱分析,发现CCAH-Ⅱ中疏水性氨基酸的摩尔分数高达42.40%,β-转角和无规卷曲结构的摩尔分数分别为30.4%、46.8%;推测高占比的疏水性氨基酸、β-转角和无规卷曲结构与小分子阿胶的高抗氧化性密切相关。Colla Corii Asini was hydrolyzed with alkaline protease,and the enzymatic hydrolysate was ultrafiltered with a 3k ultrafiltration membrane to afford a Colla Corii Asini hydrolysate(CCAH-Ⅱ).DPPH,ABTS and OH radical scavenging activities of CCAH-Ⅱat a concentration of 2 mg/mL were(53.79±1.18)%,(70.53±0.65)%and(80.00±0.45)%,respectively.By means of amino acid composition analysis and circular dichroism chromatography,it was found that the content of hydrophobic amino acids in CCAH-Ⅱwas as high as 42.40%.The proportions ofβ-turn and random coil moieties were 30.4%and 46.8%,respectively,and this high proportion ofβ-turn and random coil may be responsible for the high antioxidant activity of CCAH-Ⅱ.

关 键 词：阿胶 水解物 酶解 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]