Carboxypeptidase A6 Promotes the Proliferation and Migration of Hepatocellular Carcinoma by Up-regulating AKT Signaling Pathway  

作　　者：Qi-bo HUANG Hong-wei ZHANG Zhi-bin LIAO 

机构地区：[1]Department of Clinical Medicine,Medical College of Wuhan University of Science and Technology,Wuhan 430081,China [2]Hepatic Surgery Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

出　　处：《Current Medical Science》2019年第5期727-733,共7页当代医学科学（英文）

基　　金：supported by the State Key Project on Infectious Diseases of China(No.2018ZX10723204-003);the National Natural Science Foundation of China(No.81572855,No.81572427);the Graduates’Innovation Fund,Huazhong University of Science and Technology(No.5003540055).

摘　　要：Summary:Hepatocellular carcinoma(HCC)has a poor treatment prognosis and high mortality worldwide.Understanding the molecular mechanism underlying HCC development would benefit the identification of diagnostic biomarkers and the improvement of the treatment strategies.The expression of carboxypeptidase A6(CPA6)has been reported in epilepsy and febrile seizures rather than in any type of cancers.However,the function of CPA6 expression in HCC is not yet understood.In this study,we aimed to investigate the clinicopathological significance of the expression of CPA6 in HCC and the underlying mechanisms.We observed that the expression of the CPA6 protein was increased significantly in HCC tissues than in paracancerous tissues.To explore its function in HCC,both gain-and loss-of-function studies demonstrated that CPA6 played a vital role in promoting HCC growth and metastasis.When knocking down CPA6 with shRNA,HCC cell proliferation and migration could be suppressed.Meanwhile,CPA6 overexpression could promote proliferation and migration of HLF cells.Moreover,CPA6 could activate AKT serine/threonine kinase(AKT)signaling pathway as confirmed by Western blotting.In conclusion,our study revealed that CPA6 could promote HCC cell proliferation and migration via AKT-mediated signaling pathway.These findings suggest that CPA6 is a promising diagnostic biomarker and therapeutic target to improve the prognosis of HCC.

关 键 词：CARBOXYPEPTIDASE A6 HEPATOCELLULAR carcinoma PROLIFERATION MIGRATION AKT 

分 类 号：R73[医药卫生—肿瘤]