复合酶法提取石花菜粗多糖工艺的响应面优化  

Optimization of multi-enzymatic extraction of polysaccharide from Gelidium amansii by response surface methodology

作　　者：裴若楠 翟红蕾[1] 戚勃[1] 杨贤庆[1] PEI Ruonan;ZHAI Honglei;QI Bo;YANG Xianqing(National R&D Center for Aquatic Product Processing,Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院南海水产研究所,国家水产品加工技术研发中心,农业农村部水产品加工重点实验室,广东广州510300 [2]上海海洋大学食品学院,上海201306

出　　处：《南方水产科学》2019年第6期88-95,共8页South China Fisheries Science

基　　金：中国水产科学研究院基本科研业务费专项资金(2017GH15);广东省海洋与渔业发展专项资金项目(2017A0001);现代农业产业技术体系建设专项资金(CARS-50);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2016TS05,2016TS36,2019TS15)

摘　　要：为优化复合酶法[m(木瓜蛋白酶)∶m(纤维素酶)=2∶1]提取石花菜(Gelidium amansii)粗多糖的工艺条件,在单因素实验基础上进行三因素三水平的Box-Benhnken中心组合实验,并根据回归分析确定石花菜粗多糖的复合酶法最佳提取工艺为复合酶添加量2.30%、酶解温度60.43℃、料液比1∶32(g·mL^-1)。在此条件下酶解120 min,石花菜粗多糖的提取率可达15.98%。To optimize the multi-enzymatic extraction process of polysaccharides from Gelidium amansii,we designed a threefactor and three-level Box-Benhnken center-united experiment based on single factor test,and conducted a regression analysis.The optimum technological parameters of enzymatic extraction of G.amansii polysaccharide were:enzymes addition 2.30%,enzymatic hydrolysis temperature 60.43℃,ratio of material to water 1∶32(g·mL^-1).The extraction yield of polysaccharide was up to 15.98%after 120 min of enzymatic hydrolysis under these conditions.

关 键 词：石花菜 粗多糖 复合酶 响应面法 提取优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]