黄酒和米酒中氨基酸态氮测定方法的比较  

Comparative study on methods for determination of amino acid nitrogen in Huangjiu and Mijiu

作　　者：王斯维 王栋[1] 陶玲玲 徐岩 WANG Siwei;WANG Dong;TAO Lingling;XU Yan(School of Biotechnology,Jiangnan University,The Key Laboratory of Industrial Biotechnology,Ministry of Education,State Key Laboratory of Food Science&Technology,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院教育部工业生物技术重点实验室食品科学与技术国家重点实验室

出　　处：《食品与发酵工业》2019年第23期232-238,共7页Food and Fermentation Industries

基　　金：国家重点研发计划项目(2018YFC1604106);国家轻工技术与工程一流学科自主课题(LITE2018-12);江苏高校品牌专业建设工程资助项目(TAPP)

摘　　要：为了探究茚三酮比色法在黄酒和米酒氨基酸态氮测定中的适用性,首先对可能影响茚三酮比色法测定结果的黄酒和米酒特性因素进行了考察分析,然后与甲醛滴定法及氨基酸分析仪进行比较,对17个黄酒和米酒样品进行了测定分析。结果表明,黄酒和米酒的色泽、总酸和酒精度等特性,对茚三酮比色法的测定结果无显著影响。茚三酮比色法与甲醛滴定法测定结果之间具有较好的线性关系,但对于米酒等氨基酸态氮含量较低的样品,茚三酮比色法具有更高的精密度和准确性。对照氨基酸分析仪分析结果,甲醛滴定法更适于表征产品的总体氨基酸含量,而茚三酮比色法则能更准确反映发酵过程中的α-氨基酸含量,且对于氨基酸态氮含量较低的米酒产品测定结果相对更为准确。The applicability of ninhydrin colorimetric method for amino-acid nitrogen(AAN)measurement from Huangjiu and Mijiu analysis was determined in this work.First,factors of Huangjiu and Mijiu that may affect ninhydrin colorimetry result were investigated.Next,ninhydrin colorimetry,formaldehyde titration,and the amino acid analyzer were used for AAN measurement of 17 Huangjiu and Mijiu samples.The results showed that color,total acid,and alcohol content of Huangjiu and Mijiu had no significant effect on the ninhydrin colorimetry determination.There was a good linear relationship between the results given by ninhydrin colorimetry and formaldehyde titration.However,the ninhydrin colorimetry had higher precision and accuracy for the samples with low amino acid nitrogen content.Compared to the result of the amino acid analyzer,the formaldehyde titration was more suitable to determine the total amino acid content.Moreover,the ninhydrin colorimetry was able to accurately determine theα-amino acid content during the fermentation process.These results not only provide an alternative analysis method for amino acid nitrogen in Huangjiu,but also establish basis for the developing amino acid nitrogen analysis index in Mijiu industry.

关 键 词：黄酒 米酒 茚三酮比色法 甲醛滴定法 氨基酸态氮 

分 类 号：R28[医药卫生—中药学]