高效液相色谱法测定葛仙米水溶性多糖含量  

Determination of Nostoc Sphaeroides kützing water-soluble polysaccharide content by HPLC

作　　者：苏攀峰 唐庆九[1] 陈盛 赵立彬 王玉兰 刘艳芳[1] 周帅[1] 许剑锋[2,3] SU Panfeng;TANG Qingjiu;CHEN Sheng;ZHAO Libin;WANG Yulan;LIU Yanfang;ZHOU Shuai;XU Jianfeng(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,P.R.China,National Engineering Research Center of Edible Fungi\National R&D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;College of Food Science,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation,Shanghai 201306,China;Huhan Yandi Bioengineering CO.,LTD.,Zhuzhou 412000,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海201403 [2]上海海洋大学食品学院,上海201306 [3]上海水产品加工及贮藏工程技术研究中心,上海201306 [4]湖南炎帝生物工程有限公司,株洲412000

出　　处：《上海农业学报》2020年第1期105-110,共6页Acta Agriculturae Shanghai

摘　　要：通过建立高效液相法(HPLC)测定葛仙米水溶性多糖含量的方法,对HPLC测定葛仙米水溶性多糖含量的方法学进行考察。以纯化的葛仙米多糖为标准品,应用HPLC对经过不同预处理的葛仙米水溶性多糖进行含量测定。结果表明:选择柱型为TSKgel G6000 PWXL液相色谱分析柱的效果较好,标准品的定量上、下限分别为10mg/mL和0.078625mg/mL;标准溶液浓度(Y)在0.15625~5mg/mL范围内,峰面积与进样量(X)呈良好的线性关系,线性方程Y=1995695.548X-170.597、R^2=0.999;精密度、重复性和稳定性的RSD分别为1.59%、1.13%、2.56%,平均加样回收率为97.8%、RSD为1.72%,说明该方法灵敏度高、重复性和稳定性较好,试验结果可靠,所建方法适用于葛仙米水溶性多糖含量的测定。A HPLC(High Performance Liquid Chromatography)method for the determination of Nostoc sphaeroids kiitzing water-soluble polysaccharide content was developed.Using the purified Nostoc sphaeroids kiitzing water-soluble polysaccharide as standard,the content of water-soluble polysaccharides from Nostoc sphaeroids kiitzing after different pretreatment was determined by HPLC.The results indicated that the analytical column of TSKgel G6000 PWXL is better.The standard upper and lower bound were 10 mg/mL and 0.078625 mg/mL,the linear equation was F=1995695.548X-170.597,R^2=0.999;The precision,rrepeatability and stability of the relative standard deviation(RSD)were 1.59%,1.13%and 2.56%,the average recovery rate was 97.8%and RSD was 1.72%,indicating that the method has high sensitivity,repeatability and stability.Thus HPLC method is suitable for determining Nostoc sphaeroids kiitzing water-soluble polysaccharide content.

关 键 词：葛仙米 水溶性多糖 高效液相色谱法 含量 

分 类 号：S968.4[农业科学—水产养殖] TS201.2[农业科学—水产科学]