五种寒地果树果实的多酚含量、抗氧化活性及抗α淀粉酶活性分析  

Analysis of Polyphenolic Content,Antioxidant Capacity andα-amylase Inhibitory Activity of Five Fruits

作　　者：刘佩 谢佳璇 秦栋[1,2] 刘化禹 马小雯 张妍 霍俊伟[1,2] LIU Pei;XIE Jia-xuan;QIN Dong;LIU Hua-yu;MA Xiao-wen;ZHANG Yan;HUO Jun-wei(National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions,Northeast Agricultural University,Harbin 150030,China;College of Horticulture and Landscape,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学寒地小浆果开发利用国家地方联合工程研究中心,黑龙江哈尔滨150030 [2]东北农业大学园艺园林学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2020年第4期282-288,共7页Science and Technology of Food Industry

基　　金：国家重点研发计划项目(2016YFC500304-05);黑龙江省自然科学基金(C2017020);国家自然基金面上项目(31272130);国家自然基金青年项目(31801553)

摘　　要：为了探究寒地果树资源果实中的营养价值及体外活性,本试验测定了软枣猕猴桃、黑果腺肋花楸、草原樱桃、白穗醋栗和蔓越莓五种果实的总酚、总花色苷含量,比较了其抗氧化及抗α-淀粉酶活性,并进行了相关性分析,结果表明:不同寒地果实的总酚、总花色苷含量之间存在显著差异(P<0.05)。总酚含量为13.21~66.43 mg GAE/100 g,其中黑果腺肋花楸含量最高,白穗醋栗次之;总花色苷含量为0.15~46.45 mg C3G/100 g,黑果腺肋花楸含量最高,草原樱桃次之。另外5种果实的抗氧化能力也有所差异。用DPPH法、ABTS法、ORAC法、FRAP法测得白穗醋栗的抗氧化活性最强。用高通量淀粉浊度法测得白穗醋栗的抗α-淀粉酶活性最强,IC50为0.122 mg/m L,高于阿卡波糖(0.16 mg/m L),其AE值为2034.18 mmol AE/g,高于阿卡波糖(1550 mmoL AE/g),且是其他果实的42~127倍。相关性分析发现五种寒地果实的总酚、总花色苷含量和抗氧化活性之间存在极显著正相关(P<0.01),而与抗α-淀粉酶活性之间无显著性相关关系。To explore the nutritional value and in vitro activity of fruit grown in the cold region.The total phenolic content and total anthocyanin content of five fruits( kiwiberry,black chokeberry,sour cherry,white currant and cranberry in the cold region) were determined,the antioxidant capacity and α-amylase inhibitory activity were compared,and their correlationship was analyzed.The results showed that there were significant differences in total phenolic content and total anthocyanin content in different fruits( P < 0.05). The total phenolic content of five fruits ranged from 13.21 to 66.43 mg GAE/100 g,and the content of black chokeberry was the highest,following by white currant.The total anthocyanin content of five fruits ranged from0.15 to 66.45 mg C3 G/100 g,and the content of black chokeberry was also the highest while sour cherry was the second. In addition,their antioxidant capacity was also different.The antioxidant capacity of the white currant was the strongest,which was determined by DPPH,ABTS,ORAC and FRAP assays. The high-throughput turbidity method showed that different fruits had different inhibitory activities against α-amylase.The white currant had the strongest inhibitory activity against α-amylase,and IC50 value was 0.122 mg/m L,which was higher than that of acarbose( 0.16 mg/m L). The AE value of white currant againstα-amylase was 2034.18 mmol AE/g,which was equivalent to the acarbose( 1550 mmol AE/g),and it was 42 to 127 times higher than that of the other three fruits( except for the black chokeberry with no inhibition). Correlationship analysis showed that there was a significant correlationship between total phenolic content,total anthocyanin content and antioxidant capacity of five fruits( P < 0.01),and no significant correlation between TPC,TAC and α-amylase inhibitory activity.

关 键 词：果实 多酚 抗α-淀粉酶活性 抗氧化活性 Person相关性分析 

分 类 号：TS201.2[轻工技术与工程—食品科学]