瑞士乳杆菌MB2-1源胞外多糖对10种益生菌生长特性的影响  

Effect of Exopolysaccharides of Lactobacillus helveticus MB2-1 on Growth Characteristics of Ten Probiotics

作　　者：黄蓉 张学亮 韩烁 周子文 莫乔雅 董明盛[1] 芮昕[1] 张秋勤[1] 陈晓红[1] 李伟[1] HUANG Rong;ZHANG Xueliang;HAN Shuo;ZHOU Ziwen;MO Qiaoya;DONG Mingsheng;RUI Xin;ZHANG Qiuqin;CHEN Xiaohong;LI Wei(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2020年第6期163-169,共7页Food Science

基　　金：中央高校基本科研业务费专项(KYLH201702,KYYJ201807,KYYZ201904);国家自然科学基金面上项目(31571818,31871771);NSFC-新疆联合基金项目(U1903108);江苏省自然科学基金项目(BK20161448);江苏省“青蓝工程”项目;南京农业大学SRT计划项目(国家级20181037051)。

摘　　要：为研究瑞士乳杆菌(Lactobacillus helveticus)MB2-1源胞外多糖(exopolysaccharides,EPS)及其组分对益生菌生长特性的影响,更好阐明L.helveticus MB2-1源EPS的益生功能,测定10种益生菌及2株有害菌在以总EPS及其分离纯化后得到最多的组分EPS-2为唯一碳源的培养基中连续培养的光密度值(OD600 nm),并利用高效液相色谱法测定10种益生菌在0~48 h中总EPS的剩余量及短链脂肪酸(short-chain fatty acids,SCFA)的生成量。结果表明,10种益生菌在以源于L.helveticus MB2-1的总EPS和EPS-2纯组分为唯一碳源的培养基中,均可以利用总EPS和EPS-2以不同的速度生长繁殖,其中屎肠球菌(Enterococcus faecium)MN-1在第11.56小时最大比生长速率(μmax)达到0.225 h^-1,而2株有害菌的生长较在普通葡萄糖培养基中受到一定程度的抑制。在所有SCFA中,乳酸较其他SCFA生成量更大,其中乳酸生成量最多的是副干酪乳杆菌(L.paracasei),在第48小时浓度可达57.6 mmol/L,较发酵之初的26.1 mmol/L提高了121.7%。该研究表明L.helveticus MB2-1源EPS可作为10种益生菌的良好碳源,也可以作为潜在的益生元,用于调节人体和动物的肠道健康。This study evaluated the effects of the exopolysaccharides(EPS)produced by Lactobacillus helveticus MB2-1 and its purified components on the growth characteristics of probiotics in order to gain further insights into the probiotic function of the EPS of L.helveticus MB2-1.The optical density(OD600 nm)values of ten probiotics and two pernicious strains continuously cultured in a medium with EPS or EPS-2,the most abundant component separated and purified from EPS,as the sole carbon source were determined.The residual amounts of total EPS and the amounts of short-chain fatty acids(SCFA)produced in 10 probiotics during 48 h of culture were measured by high performance liquid chromatography(HPLC).The results showed that the 10 probiotics could grow and reproduce at different rates in the medium with EPS or EPS-2 as the sole carbon source.Among them,Enterococcus faecium MN-1grown in EPS showed a maximum growth rate of 0.225 h^-1 at 11.56 h.The growth of the two pernicious strains was inhibited to a certain extent compared with normal glucose medium.Lactic acid was produced in larger amounts than other SCFA.L.paracasei was the most efficient producer of lactic acid,and at 48 h of fermentation,the lactic acid concentration reached 57.6 mmol/L,which increased by 121.7%as compared with 26.1 mmol/L at the beginning of fermentation.This study demonstrated that the EPS derived from L.helveticus MB2-1 is a good carbon source for the ten probiotics and can be used as potential prebiotics to regulate intestinal health in humans and animals.

关 键 词：瑞士乳杆菌MB2-1 胞外多糖 生长曲线 短链脂肪酸 益生作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]