高效液相法同时检测谷物中玉米赤霉烯酮和赭曲霉毒素A  

Simultaneous Determination of Ochratoxin A and Zearalenone in Cereals by HPLC

作　　者：李克[1,2,3] 潘丽红 罗小虎[1,2,3] 王莉 王韧[1,2,3] 邢家溧 杜志红[1,2,3] 孙冬玲 陈正行[1,2,3] LI Ke;PAN Li-hong;LUO Xiao-hu;WANG Li;WANG Ren;XING Jia-li;DU Zhi-hong;SUN Dong-ling;CHEN Zheng-xing(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;Ningbo Institute for Food Control,Ningbo 315048,Zhejiang,China;School of Marine Science,Ningbo University,Ningbo 315211,Zhejiang,China;Wuxi EL PONT RadiationTechnology CO.,Ltd.,Wuxi 214151,Zhejiang,China)

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]江南大学食品学院,江苏无锡214122 [4]宁波市食品检验检测研究院,浙江宁波315048 [5]宁波大学海洋学院,浙江宁波315211 [6]无锡爱邦辐照技术有限公司,江苏无锡214151

出　　处：《食品研究与开发》2020年第6期118-123,共6页Food Research and Development

基　　金：江苏省农业科技自主创新项目[CX(17)1003];“十三五”国家重点研发计划(2017YFC1600904);现代农业产业技术体系建设专项资金资助(CARS-02-32);全国粮食行业青年拔尖人才项目(LQ2018302)。

摘　　要：该研究开发一种快速、灵敏同时检测玉米、小麦和稻米中玉米赤霉烯酮(zearalenone,ZEN)和赭曲霉毒素A(ochratoxin A,OTA)的高效液相色谱检测方法。将样品采用乙腈/水(80/20,体积比),200 r/min,30℃振荡提取30 min后,经Oasis PRiME HLB固相萃取柱净化后,上样检测。该方法ZEN和OTA检测限(limit of detection,LOD)为3.7μg/kg和0.11μg/kg,定量限(quantification Limit,LOQ)为12.25μg/kg和0.38μg/kg,线性范围分别为10μg/kg^2000μg/kg和0.2μg/kg^200μg/kg,加标样品中不同浓度的ZEN和OTA回收率为83.0%~101.3%,日内精密度和日间精密度分别为3.12%~7.03%和3.57%~9.3%。该方法适用于玉米、小麦和稻米中ZEN和OTA的同时检测。A sensitive and rapid method had been developed for the simultaneous determination of ochratoxin A(OTA)and zearalenone(ZEN)in corn,wheat and rice by high performance liquid chromatography(HPLC).After extraction with acetonitrile and water(80/20,volume ratio)for 30 min under 30°C and 200 r/min in a rotary shaker,the cereal samples were purified using Oasis PRiME HLB cartridge.The limits of detection and quantification of the mycotoxins were determined under the optimum HPLC conditions at 3.7μg/kg and12.25μg/kg for ZEN and 0.11μg/kg and 0.38μg/kg for OTA,respectively.High correlation coefficients(R2>0.99)were obtained in the range of 10μg/kg-2000μg/kg for ZEN and 0.2μg/kg-200μg/kg for OTA.The average recoveries ranged from 83.0%to 101.3%for different concentrations of ZEN and OTA in spiked samples along with good intra-day(3.12%-7.03%)and inter-day(3.57%-9.3%)precision.The method proposed is suitable for the simultaneous determination of ZEN and OTA and can be performed for the mycotoxin analysis in corn,wheat and rice.

关 键 词：玉米赤霉烯酮 赭曲霉毒素A 高效液相色谱 检测 谷物 

分 类 号：O657.72[理学—分析化学] TS210.7[理学—化学]