Mechanistic target of rapamycin kinase(Mtor)is required for spermatogonial proliferation and differentiation in mice  

作　　者：Jun Cao Zuo-Bao Lin Ming-Han Tong Yong-Lian Zhang Yi-Ping Li Yu-Chuan Zhou 

机构地区：[1]State Key Laboratory of Molecular Biology,Shanghai Key Laboratory of Molecular Andrology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China [2]State Key Laboratory of Cell Biology,Shanghai Key Laboratory of Molecular Andrology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Asian Journal of Andrology》2020年第2期169-176,共8页亚洲男性学杂志（英文版）

基　　金：This work was supported by the Ministry of Science and Technology of the People’s Republic of China(NO.2014CB943103);the National Natural Science Foundation of China(No.31671203 and NO.31471104);the Science and Technology Commission of Shanghai Municipality(NO.17JC1420100).

摘　　要：Spermatogonial development is a vital prerequisite for spermatogenesis and male fertility.However,the exact mechanisms underlying the behavior of spermatogonia,including spermatogonial stem cell(SSC)self-renewal and spermatogonial proliferation and differentiation,are not fully understood.Recent studies demonstrated that the mTOR complex 1(mTORC1)signaling pathway plays a crucial role in spermatogonial development,but whether MTOR itself was also involved in any specific process of spermatogonial development remained undetermined.In this study,we specifically deleted Mtor in male germ cells of mice using Stra8-Cre and assessed its effect on the function of spermatogonia.The Mtor knockout(KO)mice exhibited an age-dependent perturbation of testicular development and progressively lost germ cells and fertility with age.These age-related phenotypes were likely caused by a delayed initiation of Mtor deletion driven by Stra8-Cre.Further examination revealed a reduction of differentiating spermatogonia in Mtor KO mice,suggesting that spermatogonial differentiation was inhibited.Spermatogonial proliferation was also impaired in Mtor KO mice,leading to a diminished spermatogonial pool and total germ cell population.Our results show that MTOR plays a pivotal role in male fertility and is required for spermatogonial proliferation and differentiation.

关 键 词：male fertility MICE MTOR SPERMATOGENESIS SPERMATOGONIA TESTIS 

分 类 号：R697[医药卫生—泌尿科学]