Reduced semen quality in patients with testicular cancer seminoma is associated with alterations in the expression of sperm proteins  

作　　者：Tania R Dias Ashok Agarwal Peter N Pushparaj Gulfam Ahmad Rakesh Sharma 

机构地区：[1]American Center for Reproductive Medicine,Cleveland Clinic,Cleveland,OH 44195,USA [2]Department of Health Sciences,Faculty of Health Sciences,University of Beira Interior,Covilha 6201-001,Portugal [3]Department of Microscopy and Unit for Multidisciplinary Research in Biomedicine,Institute of Biomedical Sciences Abel Salazar,University of Porto,Porto 4050-313,Portugal [4]Center of Excellence in Genomic Medicine Research,Faculty of Applied Medical Sciences,Jeddah 21589,Saudi Arabia [5]Division of Pathology,School of Medical Sciences,Sydney University,Lidcombe NSW 2141,Australia

出　　处：《Asian Journal of Andrology》2020年第1期88-93,共6页亚洲男性学杂志（英文版）

基　　金：Financial support for this study was provided by the American Center for Reproductive Medicine,Cleveland Clinic,OH,USA;Tania R Dias was supported by the Portuguese Foundation for Science and Technology(FCT,SFRH/BD/109284/2015);Fulbright Program(E0585639).

摘　　要：Testicular cancer seminoma is one of the most common types of cancer among men of reproductive age.Patients with this condition usually present reduced semen quality,even before initiating cancer therapy.However,the underlying mechanisms by which testicular cancer seminoma affects male fertility are largely unknown.The aim of this study was to investigate alterations in the sperm proteome of men with seminoma undergoing sperm banking before starting cancer therapy,in comparison to healthy proven fertile men(control group).A routine semen analysis was conducted before cryopreservation of the samples(n=15 per group).Men with seminoma showed a decrease in sperm motility(P=0.019),total motile count(P=0.001),concentration(P=0.003),and total sperm count(P=0.001).Quantitative proteomic analysis identified 393 differentially expressed proteins between the study groups.Ten proteins involved in spermatogenesis,sperm function,binding of sperm to the oocyte,and fertilization were selected for validation by western blot.We confirmed the underexpression of heat shock-related 70 kDa protein 2(P=0.041),ubiquinol-cytochrome C reductase core protein 2(P=0.026),and testis-specific sodium/potassium-transporting ATPase subunit alpha-4(P=0.016),as well as the overexpression of angiotensin I converting enzyme(P=0.005)in the seminoma group.The altered expression levels of these proteins are associated with spermatogenesis dysfunction,reduced sperm kinematics and motility,failure in capacitation and fertilization.The findings of this study may explain the decrease in the fertilizing ability of men with seminoma before starting cancer therapy.

关 键 词：male FERTILITY PROTEOMICS SEMINOMA SPERM PROTEINS SPERM quality TESTICULAR cancer 

分 类 号：R737.21[医药卫生—肿瘤]