培养基成分和培养条件对出芽短梗霉ZUST-I13产聚苹果酸的影响  

Effects of medium ingredients and culture conditions on the production of poly-malic acid by Aureobasidium pullulans ZUST-I13

作　　者：章雨卉 吕磊磊 陈林杰 魏培莲[1] ZHANG Yuhui;L Leilei;CHEN Linjie;WEI Peilian(School of Biological&Chemical Engineering,Zhejiang University of Science&Technology,Hangzhou 310023,China)

机构地区：[1]浙江科技学院生物与化学工程学院,浙江杭州310023

出　　处：《发酵科技通讯》2020年第1期7-10,共4页Bulletin of Fermentation Science and Technology

基　　金：浙江省自然科学基金项目(LY15C200014);浙江省大学生科技创新活动计划(新苗人才计划)项目(2018R415016)。

摘　　要：聚苹果酸是出芽短梗霉(Aureobasidium pullulans)菌株产生的一种高分子聚合物,由于其良好的水溶性、生物相容性、可降解性等特性,在食品、医药、化妆品等领域具有广泛的应用前景。聚苹果酸的合成受到多种营养成分以及环境因素的影响。采用单因素实验法研究了常见培养基成分和培养条件对出芽短梗霉菌株ZUST-I13发酵产聚苹果酸的影响。结果表明:葡萄糖和尿素相较于其他的碳、氮源有利于聚苹果酸的生产;酸性环境(初始pH 3~4)适宜出芽短梗霉发酵产聚苹果酸;发酵温度25℃适合聚苹果酸的生产;金属离子Zn^2+对出芽短梗霉产聚苹果酸有一定的促进作用,在Zn^2+质量浓度为0.05 g/L时聚苹果酸产量最大。With excellent water solubility,biocompatibility and degradability,poly-malic acid(PMA),a kind of high-molecular polymer produced by Aureobasidium pullulans,could be used in food,pharmaceutical and cosmetic industries widely.In present research,the effects of medium ingredients and culture conditions on Aureobasidium pullulans ZUST-I13 were explored.These results demonstrated that glucose was suitable carbon source and urea was suitable nitrogen source for producing PMA respectively.It showed that acidic condition(initial pH 3-4)and culture temperature of 25℃ were favorable for the production of PMA.And addition Zn^2+also could promote the production of poly-malic acid.The highest yield was obtained when the Zn^2+concentration was 0.05 g/L.

关 键 词：出芽短梗霉 聚苹果酸 发酵 培养条件 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]