槐耳醇提物的抗氧化降血糖活性  

Antioxidant and Hypoglycemic Activity of Ethanol Extract from Trametes robiniophila

作　　者：苏伟 陈盛芳 施昊卿 宋康馨 李超[1] SU Wei;CHEN Shengfang;SHI Haoqing;SONG Kangxin;LI Chao(College of Food Engineering,Xuzhou Institute of Technology,Xuzhou 221018)

机构地区：[1]徐州工程学院食品工程学院,徐州221018

出　　处：《食品工业》2020年第4期168-171,共4页The Food Industry

基　　金：国家科技富民强县专项行动计划项目(BN2012102);江苏省苏北科技发展计划项目(BN2015018);徐州工程学院培育项目(XKY2018137)。

摘　　要：采用分光光度计法对槐耳醇提物进行成分分析,采用四种方法对其抗氧化性进行评估,并探讨其对α-淀粉酶的抑制作用。结果表明:槐耳醇提物中多酚含量为103.62 mg PE/g FDW(Pyrogallol equivalent,PE;Freeze drying weight,FDW),黄酮含量为195.64 mg RE/g FDW(Rutin equivalent,RE),黄酮醇含量为1.50 mg RE/g FDW,黄烷醇含量为203.2 mg CE/g FDW(Catechin equivalent,CE),酚酸含量为43.79 mg PE/g FDW;通过铁离子还原抗氧化能力、清除ABTS+自由基能力、总还原力和总抗氧化力四种方法得出槐耳醇提物具有较强的抗氧化活性;槐耳醇提物抑制α-淀粉酶的IC50值为1.28 mg/mL,表明其具有高效的降血糖的能力。The composition of the ethanol extract of Trametes robiniophila was analyzed by spectrophotometer.Four methods were used to evaluate the antioxidant activity of ethanol extract from Trametes robiniophila and the inhibition ofα-amylase was discussed.The results showed that the polyphenols content in Trametes robiniophila ethanol extract was 103.62 mg PE/g FDW(Pyrogallol equivalent,PE;Freeze drying weight,FDW),flavonoids content was 195.64 mg RE/g FDW(Rutin equivalent,RE),flavonol content was 1.50 mg RE/g FDW,flavanol content was 203.2 mg CE/g FDW(Catechin equivalent,CE),and phenolic acid content was 43.79 mg PE/g FDW.The antioxidant activity of the extract was obtained using ferric reducing antioxidant power,ABTS+radical scavenging assay,reducing power assay and total antioxidant capacity.The IC50 value of Trametes robiniophila ethanol extract inhibitingα-amylase was 1.28 mg/mL,indicating that it had an effective hypoglycemic activity.

关 键 词：槐耳 醇提物 抗氧化活性 降血糖活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]