Soleris检测技术在焙烤食品微生物检测中的应用研究  

Application of Soleris System in the Detection of Microorganism in Baked Food

作　　者：张雪苍 周国磊 傅科涵 王东阳 赵爱梅 揭金潮 田文秀 陈钊[2] ZHANG Xuecang;ZHOU Guolei;FU Kehan;WANG Dongyang;ZHAO Aimei;JIE Jinchao;TIAN Wenxiu;CHEN Zhao(Shaanxi Grain and Oil Science Research and Design Institute,Xi’an 710082,China;Beijing Institute of Grain Science,Beijing 101101,China)

机构地区：[1]陕西省粮油科学研究设计院,陕西西安710082 [2]北京市粮食科学研究院,北京101101

出　　处：《食品科技》2020年第4期321-326,共6页Food Science and Technology

摘　　要：应用Soleris检测技术,以实现对焙烤食品(面包)中的菌落总数和大肠菌群的快速检测,并验证其检测结果的可靠性。得到焙烤食品中菌落总数的标准曲线为lg cfu/g=-0.44DT+9.3595,R^2=0.9833;大肠菌群的标准曲线为lg cfu/g-0.7422DT+8.6369,R^2=0.9803。用平板计数法对标准曲线进行验证,结果显示,2种方法对菌落总数(大肠菌群)的测定结果间无显著差异,结果对数值的绝对差值低于0.5,具有较好的一致性;对Soleris法测定菌落总数和大肠菌群的重复性进行验证,RSD分别为1.72%~3.87%、3.27%~4.34%。相较平板计数法,Soleris法可缩短一半以上的检测时间,大幅提高检测效率。文章建立的焙烤食品中菌落总数和大肠菌群的Soleris快速检测方法,可为焙烤食品生产企业在微生物快检方法的选择上提供参考。Soleris system was applied to detect total bacterial plate count and coliforms in bread.The standard curve of total bacterial count in bread is:lg cfu/g=-0.44DT+9.3595,R^2=0.9833,while the standard curve of coliform:lg cfu/g=-0.7422DT+8.6369,R^2=0.9803.The plate counting method was used to verify the standard curve,and the results showed that there was no significant difference between two methods in total bacterial count (coliforms),and the absolute difference between the two methods was less than 0.5,showing a good consistency.Repetitive test results show that RSD value of Soleris method to determine total bacterial count and coliforms,was 1.72%~3.87% and 3.27%~4.34%,respectively.Compared with plate counting method,Soleris method could shorten the detection time by more than half and greatly improve the detection efficiency.The Soleris detection method for total bacterial count and coliforms in baked food was established in this paper,which could provide a reference for the choice of the rapid detection method of microorganism in baked food production enterprises.

关 键 词：焙烤食品 快速检测 Soleris技术 菌落总数 大肠菌群 

分 类 号：TS201.3[轻工技术与工程—食品科学]