响应面法优化水豆豉纯种发酵产大豆异黄酮的条件  

Optimization of Pure Breed Fermentation Conditions for Soybean Isoflavones Produced by Fermented Soybeans with Response Surface Methodology

作　　者：沈丹芸 董科 范紫玮 陈静娴 何迅 裴晓方 SHEN Dan-yun;DONG Ke;FAN Zi-wei;CHEN Jing-xian;HE Xun;PEI Xiao-fang(West China School of Public Health(West China Fourth Hospital),Sichuan University,Chengdu 610041,China;Key Laboratory of Food Safety Monitoring and Risk Assessment in Sichuan Province,Chengdu 610041,China)

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),成都610041 [2]食品安全监测与风险评估四川省重点实验室,成都610041

出　　处：《中国调味品》2020年第6期31-36,共6页China Condiment

摘　　要：目的:为了提高水豆豉中大豆异黄酮的最高产量,通过响应面法优化水豆豉纯种发酵产大豆异黄酮的条件。方法:利用前期筛选出来的水豆豉高效发酵菌株进行黄豆发酵,经过单因素实验得到较优的3个水平,以大豆异黄酮含量和感官评价得分为响应值,通过响应面实验获得最优发酵条件。结果:水豆豉的最优发酵条件为发酵温度40℃、发酵时间4 d、接菌量1.00%。在此条件下进行发酵,可获得最高大豆异黄酮含量和感官评价得分,分别为881.28 mg/kg和74.50分。结论:获得该菌株的最佳发酵条件,为研发高产大豆异黄酮的水豆豉积累了资料。Objective:In order to improve the highest yield of soybean isoflavones of fermented soybeans,the pure breed fermentation conditions for producing soybean isoflavones by fermented soybeans are optimized by response surface methodology.Method:The soybean fermentation is carried out by the high-efficient fermentation strains of fermented soybeans screened in the early stage,and the three optimal levels are obtained through the single factor experiment.The optimal fermentation process is then obtained by the response surface methodology with isoflavones content and sensory evaluation scores as the response values.Result:The optimum fermentation conditions of fermented soybeans are as follows:the fermentation temperature is 40℃,the fermentation time is 4 d and the inoculation amount is 1.00%.Under such conditions,the highest isoflavones content and the highest sensory evaluation score are obtained,which is 881.28 mg/kg and 74.50 points respectively.Conclusion:The best fermentation conditions of the strains are obtained,which has accumulated data for the research and development of fermented soybeans with high-yield isoflavones.

关 键 词：水豆豉 纯种发酵 响应面优化 大豆异黄酮 感官评价 

分 类 号：TS201.56[轻工技术与工程—食品科学]