弱磁场对胰蛋白酶活性和构象的影响  

Effect of Weak Magnetic Field on Trypsin Activity and Structure

作　　者：朱一帆 陈静 王晟喆 胡晓梦 邓尚贵 ZHU Yi-fan;CHEN Jing;WANG Sheng-zhe;HU Xiao-meng;DENG Shang-gui(College of Food and Medicine,Zhejiang Ocean University,Zhoushan 316022,China;Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province,Zhoushan 316000,China)

机构地区：[1]浙江海洋大学食品与医药学院,浙江舟山316022 [2]浙江省海产品健康危害因素关键技术研究重点实验室,浙江舟山316000

出　　处：《食品工业科技》2020年第14期36-40,共5页Science and Technology of Food Industry

基　　金：浙江省自然科学基金(LY17C200010);国家自然科学基金青年基金(31901764)。

摘　　要：本文采用100 mT磁场处理胰蛋白酶2 h,并利用红外光谱、圆二色谱等研究了磁场处理前后胰蛋白酶活性及结构的变化,采用抑菌圈法测定了胰蛋白酶水解梅鱼蛋白所得酶解液的抑菌活性变化。结果表明,经100 mT磁场处理2 h后单位浓度酶解液的抑菌圈直径得到提高,其中大肠杆菌(Escherichia coli)抑菌圈直径由(4.71±0.22)mm提高至(8.54±0.23)mm,酶活相较于未处理组高52.5 U/min;弱磁场处理后,胰蛋白酶氨基酸总量下降,必需氨基酸占比上升;红外光谱峰整体蓝移,α-螺旋和β-折叠含量增加。In this paper,trypsin was treated with 100 mT magnetic field for 2 h,and the changes of trypsin activity and structure before and after magnetic field treatment were studied by infrared spectrum and circular dichroism.The antimicrobial activity of enzymatic hydrolysate produced by small yellow croaker hydrolyzed by trypsin was determined by inhibition zone method.The results showed that the enzymatic hydrolysate prepared by the enzyme treated with 100 mT magnetic field for 2 h had an increasing inhibition zone diameter,the inhibition zone diameter of Escherichia coli increased from(4.71±0.22)mm to(8.54±0.23)mm,and the enzyme activity increased by 52.5 U/min,compared with the untreated group.The change in the structure of trypsin was that the total amount of amino acids decreased,the proportion of essential amino acids increased,the peaks of the infrared spectrum were blue-shifted,and the proportion ofα-helix andβ-flod increased.

关 键 词：弱磁场 胰蛋白酶 梅鱼 酶解液 活性 构象 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]