高效液相色谱法同时测定香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇  

Simultaneous Detection of L-phenylalanine and 2-phenylethanol in Hypoxylon sp.Fermented Liquid by HPLC

作　　者：杨立志 林隆基 宋燕娇 杨娟 邓优锦[2] 江玉姬[1,2] YANG Li-zhi;LIN Long-ji;SONG Yan-jiao;YANG Juan;DENG You-jin;JIANG Yu-ji(College of Food Science,Fujian Agriculture and Forestry Uneiversity,Fuzhou 350002,China;Mycological Research Center,Fujian Agriculture and Forestry Uneiversity,Fuzhou 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002

出　　处：《食品工业科技》2020年第14期210-215,共6页Science and Technology of Food Industry

基　　金：福建农林大学创新基金项目(KF2015050)。

摘　　要：为了检测香灰菌发酵液中L-苯丙氨酸的利用情况和2-苯基乙醇的生成量,建立了一种利用HPLC外标法同时检测香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇的方法。方法色谱条件为:色谱柱:C18(5μm,250 mm×4.6 mm);柱温30℃;进样量10μL;流速0.7 mL/min;DAD检测器,检测波长258 nm;流动相A:0.6%乙酸水溶液;流动相B:甲醇。结果表明:两种物质的浓度分别在1~5和1.02~5.1 g/L范围内与峰面积有良好的线性关系,相关系数r均大于0.999,L-苯丙氨酸检出限和定量限分别为1.023和3.409 mg/L,2-苯基乙醇检出限和定量限分别为0.567和1.890 mg/L,L-苯丙氨酸加标回收率为99.16%~101.58%,RSD(n=6)为0.67%,2-苯基乙醇的加标回收率为98.53%~101.06%,RSD(n=6)为1.22%。该方法操作简便快速、准确度和灵敏度高,可满足对香灰菌发酵液中L-苯丙氨酸和2-苯基乙醇风味物质的同时检测。In order to simultaneously detect the usage of L-phenylalanine and the production of 2-phenyl-ethanol in Hypoxylon sp.fermented liquid,an HPLC external standard method was developed.The method chromatographic conditions were as follows:Chromatographic column:C18(5μm,250 mm×4.6 mm),Column temperature 30℃,the injection volume 10μL,flow rate 0.7 mL/min,DAD detector,detection wavelength 258 nm,mobile phase A 0.6% acetic acid solution,mobile phase B methanol.The results indicated that good linearities between concentration and peak area were exist for L-phenylalanine and 2-phenylethanol with correlation coefficients greater than 0.999 in the range of 1~5 and 1.02~5.1 g/L,respectively.The detection limit and the quantitative limit of L-phenylalanine were 1.023 and 3.409 mg/L,respectively.The detection limit and the quantitative limit of 2-phenylethanol were 0.567 and 1.890 mg/L,respectively.The spiked recoveries of L-phenylalanine were in the range of 99.16%~101.58% with relative standard deviations(n=6)of 0.67%.The spiked recoveries of 2-phenylethanol were in the range of 98.53%~101.06% with relative standard deviations(n=6)of 1.22%.The method was simple width high accuracy and sensitivity.It was suitable for simultaneously determining the L-phenylalanine and 2-phenylethanol in the Hypoxylon sp.fermented liquid.

关 键 词：香灰菌 发酵液 L-苯丙氨酸 2-苯基乙醇 同时测定 高效液相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]