生食大眼金枪鱼中生物胺产生菌的分离与鉴定  

Isolation and identification of biogenic amine-producing bacteria from raw bigeye tuna( Thunnus obesus)

作　　者：李少丽 邓建朝[1] 李春生[1] 杨贤庆[1] 吴燕燕[1] 陈胜军[1] 马海霞[1] LI Shaoli;DENG Jianchao;LI Chunsheng;YANG Xianqing;WU Yanyan;CHEN Shengjun;MA Haixia(Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院南海水产研究所,农业农村部水产品加工重点实验室,广东广州510300 [2]上海海洋大学食品学院,上海201306

出　　处：《食品与发酵工业》2020年第14期121-126,共6页Food and Fermentation Industries

基　　金：国家重点研发计划资助(2017YFC1600706,2018YFF0213605);国家现代农业(海水鱼)产业技术体系建设专项(CARS-47);国家水产品质量安全风险评估(GJFP2019032);广东省现代农业产业技术体系创新团队建设专项(2019KJ151);海水产品品质评价与调控创新团队项目(2020TD73)。

摘　　要：为了探究大眼金枪鱼(Thunnus obesus)生鱼片中生物胺产生菌的产胺特性,从市售大眼金枪鱼生鱼片中分离出9株生物胺产生菌的单菌落并确定其生长曲线,采用高效液相色谱法分析了菌株发酵液中生物胺的浓度。结果表明,9株菌株为嗜温菌,适宜环境下均可产生色胺、腐胺和尸胺。筛选出3株生物胺产量较高的菌株,通过单菌落接种于TSB培养基培养,3 d累积产生生物胺的质量浓度分别为40.4、41.3和50.3 mg/L。将产胺量较高的菌株进行VITEK 2和16S r DNA鉴定,3株菌株分别为溶酪巨型球菌(Macrococcus caseolyticus),产碱普罗威登斯菌(Providencia alcalifaciens)和粪肠球菌(Enterococcus faecalis)。该研究结果为进一步研究如何抑制大眼金枪鱼中生物胺的产生提供了理论依据,也为大眼金枪鱼的保藏研究奠定了理论基础。To investigate the characteristics of amine-producing bacteria from raw bigeye tuna( Thunnus obesus),total nine strains of biogenic amine-producing bacteria were isolated from the commercial bigeye tuna,and their growth curves were also determined. The concentrations of biogenic amines in the fermented broth were determined by high performance liquid chromatography. The results showed that all nine strains belong to mesophilic bacteria,which could produce tryptamine,putrescine and cadaverine under suitable conditions. Three strains with the highest biogenic amine yield were selected. By inoculation the single colony into tryptic soy broth,the cumulative mass concentrations of biogenic amines induced by these strains over 3 days were 40. 4,41. 3 and 50. 3 mg/L,respectively.Identification of these three strains performed by VITEK 2 and 16 S r DNA showed that they were Macroococcus caseolyticus,Providencia alcalifaciens,and Enterococcus faecalis,respectively. The results provide an important theoretical basis for further study on inhibiting the production of biogenic amines in raw bigeye tuna,and also lay an important theoretical foundation for the preservation research of raw bigeye tuna.

关 键 词：大眼金枪鱼 生鱼片 产胺菌 分离 鉴定 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]