植物乳杆菌ZJ316培养基优化和高密度培养的研究  

Optimization of Fermentation Medium and the High-density Culturing Conditions for Lactobacillus plantarum ZJ316

作　　者：陈百莹 郑苗[1] 邓泽元[1] 李红艳[1] Chen Baiying;Zheng Miao;Deng Zeyuan;Li Hongyan(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047)

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《中国食品学报》2020年第7期65-74,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：江西省杰出青年人才资助计划项目(20162BCB23018)。

摘　　要：针对植物乳杆菌ZJ316,通过单因素和响应面试验设计法,对其培养基配方进行优化。同时,对植物乳杆菌ZJ316高密度培养条件、菌体收集条件和分批补料方法进行研究。结果表明,植物乳杆菌ZJ316优化培养基(质量分数)为:葡萄糖2.71%,酵母粉0.45%,牛肉膏0.6%。最佳培养条件为:培养基初始pH 6,接种量4%,摇床转速80 r/min,培养温度35℃。在离心条件为4000 r/min,20 min时,植物乳杆菌菌体成活率达到最大值为81.09%。此外,采用间歇补料的方法,为增大菌体数可适当添加氮源,菌体数可达9.28×10^9CFU/mL。The condition of Lactobacillus plantarum formulation was optimized by the single factor and response surface methodology.Meanwhile,the conditions of high-density cultivation,collection of cells and fed batch fermentation method were studied.The results showed that the optimized medium was 2.71% of glucose,yeast 0.45% and beef extract0.6%.The optimal cultivation conditions were as follows:initial pH 6,inoculation amount 4%,shaking speed 80 r/min,cultivation temperature 35 ℃.Under the conditions of 4 000 r/min and 20 min,Lactobacillus plantarum collection was at the highest survival rate,reaching 81.09%.In addition,the proper addition of nitrogen source can promote the proliferation of the cells,which could reached 9.28 × 10~9 CFU/mL.

关 键 词：植物乳杆菌 响应面 高密度培养 补料分批培养 

分 类 号：TS201.3[轻工技术与工程—食品科学]