超声辅助提取盐碱地苦菜多酚及其抗氧化活性研究  

Study on the ultrasonic assisted extraction technology and antioxidant activity of total polyphenols from Sonchus oleraceus in saline-alkali soil

作　　者：王红玉[1] 焦林宏[1] 赵芳[1] 本莲芳 WANG Hong-yu;JIAO Lin-hong;ZHAO Fang;BEN Lian-fang(College of Petroleum Chemical Engineering,Lanzhou Petrochemical College of Vocational Technology,Lanzhou 730060,Gansu,China)

机构地区：[1]兰州石化职业技术学院石油化学工程学院,甘肃兰州730060

出　　处：《粮食与油脂》2020年第7期74-78,共5页Cereals & Oils

基　　金：兰州石化职业技术学院校级项目(KJ2018-09)。

摘　　要：以总多酚提取率为指标,采用单因素及正交试验优化苦菜多酚的提取工艺条件;以自由基清除率为指标,用抗坏血酸作对照品,研究苦菜多酚的抗氧化活性。结果表明,最佳提取工艺条件为超声功率为150 W、乙醇浓度60%、超声温度55℃、超声时间40 min、料液比1∶50(g/mL)、提取3次。在该工艺条件下,苦菜多酚的提取率为65.3 mg/g。苦菜多酚对DPPH·清除作用的IC 50值为1.44μg/mL,对·OH清除作用的IC 50值为83.72μg/mL,其清除能力均强于抗坏血酸,表明盐碱地苦菜多酚有较好的抗氧化活性。With the extraction rate of total polyphenols as index,the optimum extraction conditions of polyphenols from Sonchus oleraceus were optimized by single factor and orthogonal experiments.With scavenging rate of free radical as the index,the antioxidant activity of polyphenols was studied with ascorbic acid as the reference substance.Results showed that the optimum technology conditions were as follows:150 W of ultrasonic power,60%of ethanol concentration,55℃of ultrasonic temperature,40 min of ultrasonic time,1∶50(g/mL)of solid-liquid ratio,and the extraction was performed for 3 times.The extraction solution of polyphenol was obtained.Under these conditions,the extraction rate of polyphenols from Sonchus oleraceus was 65.3 mg/g.The IC50 of DPPH·scavenging capacity of polyphenols was 1.44μg/mL,and the IC50 of·OH scavenging capacity was 83.72μg/mL,both of which were stronger than ascorbic acid,indicating that polyphenols from Sonchus oleraceus in salinealkali soil have better antioxidant activity.

关 键 词：苦菜 多酚 提取 自由基 

分 类 号：TS201.2[轻工技术与工程—食品科学]