嗜热链球菌MN-ZLW-002体外益生特性与安全性分析  

The Probiotic Properties in Vitro and Safety Analysis of Streptococcus thermophiles MN-ZLW-002

作　　者：康小红 王凡 陈建国 韩雪[1] 张兰威[1] KANG Xiao-hong;WANG Fan;CHEN Jian-guo;HAN Xue;ZHANG Lan-wei(School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Harbin 150090,China;Inner Mongolia Meng Nin Dairy Industry(Group)Co.,Ltd.,R&D Center,Hohhot 011500,China)

机构地区：[1]哈尔滨工业大学化工与化学工程学院,黑龙江哈尔滨150090 [2]内蒙古蒙牛乳业(集团)股份有限公司研发中心,内蒙古呼和浩特011500

出　　处：《食品工业科技》2020年第15期124-128,共5页Science and Technology of Food Industry

基　　金：国家重点研发计划(政府间国际科技创新合作,中澳乳品未来技术联合研究中心,2016YFE0101200)。

摘　　要：本研究对分离甘南地区的嗜热链球菌MN-ZLW-002进行了体外益生功能和安全性分析。结果显示,MN-ZLW-002对p H2.0及pH3.0的人工胃液耐受性较好,但对0.3%胆盐条件耐受性较弱;菌株MN-ZLW-002对胆固醇的降解率为42.66%±2.11%,对氯霉素、庆大霉素、链霉素、四环素、万古霉素、卡那霉素、青霉素、红霉素和克林霉素均敏感,且MN-ZLW-002急性经口毒性属无毒。综合上述结果,MN-ZLW-002具有良好的模拟胃液耐受性、较强的胆固醇降解能力,且安全无毒,可作为功能型发酵食品原料进行开发,具有巨大的商业价值。In this study,the probiotic property in vitro and safety of Streptococcus thermophilus MN-ZLW-002 isolated from Gannan area were analyzed.The results showed that MN-ZLW-002 was well tolerated to artificial gastric juice at p H2.0 and pH3.0,but weakly tolerated to 0.3%bile salt.The cholesterol degradation rate of strain MN-ZLW-002 was 42.66%±2.11%.MN-ZLW-002 was sensitive to chloramphenicol,gentamicin,streptomycin,tetracycline,vancomycin,kanamycin,penicillin,erythromycin and clindamycin.Meanwhile,MN-ZLW-002 acute transoral toxicity was nontoxic.In a conclusion,MN-ZLW-002 is not only non-toxic and has a good tolerance to simulated gastric juice,but also has a strong ability to degrade cholesterol.It can be developed as a functional fermented food raw material and has a great commercial value.

关 键 词：嗜热链球菌 耐酸耐胆盐 降胆固醇 安全性 

分 类 号：TS201.3[轻工技术与工程—食品科学]