毛蚶体内金属硫蛋白提取工艺优化  

Optimization of Extraction Technology of Metallothionein in Arca subcrenata

作　　者：孟春英[1] 王奎武[2] 李停停 张小军[1] 黄丽英[1] 顾捷[1] 陈雪昌[1] MENG Chun-ying;WANG Kui-wu;LI Ting-ting;ZHANG Xiao-jun;HUANG Li-ying;GU Jie;CHEN Xue-chang(Marine Fisheries Research Institute of Zhejiang Province,Key Laboratory of Sustainable Utilization of Technology Research for Fisliery Resource of Zhejiang Province,Zhoushan 316000,China;Zhejiang Gongshang University,Hangzhou 310000,China;Zhejiang Ocean University,Zhoushan 316000,China)

机构地区：[1]浙江省海洋水产研究所,浙江省海洋渔业资源可持续利用研究重点实验室,浙江舟山316000 [2]浙江工商大学,浙江杭州310000 [3]浙江海洋大学,浙江舟山316000

出　　处：《食品工业科技》2020年第15期173-178,187,共7页Science and Technology of Food Industry

基　　金：国家重点研发计划(2016YFF0202304);2019年浙江省科研院所转项(海水产品精深加工关键技术研究及应用示范)。

摘　　要：旨在建立一种金属硫蛋白(metallothionein,MT)的高效提取工艺。采用了Zn2+诱导毛蚶机体产生金属硫蛋白,比较诱导前后毛蚶体内MT含量的变化。探究毛蚶中金属硫蛋白的提取方法并优化提取因素,选用Tris-HCl缓冲液为提取剂。在单因素实验的基础上,选取对MT提取含量影响较大的因素包括提取液p H、提取液浓度、液料比、提取温度等进行试验。以MT提取含量作为响应值,根据Box-Behnken实验原理,在四因素三水平的基础上进行毛蚶金属硫蛋白提取工艺响应面优化分析。结果表明,最佳提取工艺参数为:液料比6∶1 m L/g,提取液pH为8.45,提取温度35℃,缓冲液浓度0.18 mol/L。在此参数条件下,MT提取量为0.695 mg/g,与模型的预测值基本相符。因此,本研究建立了从毛蚶中高效提取MT的工艺。The study was aimed to establish an efficient extraction technology of metallothionein.The present experiment was conducted to investigate the extraction of metallothionein(MT)from Arca subcrenata by Zn2+stimulated.The changes of MT content before and after induction were compared.To explore the extraction method of metallothionein from Arca subcrenata and optimize the extraction factors,Tris-HCl buffer was selected as extractant.On the basis of single factor experiments,the factors that had great influence on MT extraction content were selected,including pH of extraction buffer,concentration of buffer,ratio of material to liquid and extraction temperature.Based on Box-Behnken experiment principle,response surface optimization analysis of metallothionein extraction process was carried out on the basis of four factors and three levels with MT extraction content as response value.The results showed that the best optimum extraction conditions of MT in Arca subcrenata were as follows:The ratio of extraction buffer to Arca subcrenata tissue was 6∶1 m L/g,the concentration of extractant was 0.18 mol/L,the pH of extracti buffer was 8.45,and the heating temperature was 35℃.Under these conditions,the obtained MT was0.695 mg/g in the actual experiment which were almost accorded with the predicted data.Therefore,this study established an efficient extraction process of MT from Arca subcrenata.

关 键 词：响应面法 金属硫蛋白 提取工艺 毛蚶 

分 类 号：TS201.3[轻工技术与工程—食品科学]