螺旋筛板气升式反应器在真菌发酵中的应用  

Application of airlift reactor with helical sieve plate in fungal fermentation

作　　者：李想[1] 陈瑜琦 王子凡 郑志永[1,2] 陈海琴 高敏杰[1] 詹晓北 LI Xiang;CHEN Yuqi;WANG Zifan;ZHENG Zhiyong;CHEN Haiqin;GAO Minjie;ZHAN Xiaobei((Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China;School of Environmental and Civil Engineering,Jiangnan University,Wuxi 214122,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]糖化学与生物技术教育部重点实验室(江南大学),江苏无锡214122 [2]江南大学环境与土木工程学院,江苏无锡214122 [3]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2020年第15期93-99,共7页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(31301408);江苏省重点研发计划(现代农业)(BE2018367)。

摘　　要：在发酵工业中,性能优良的反应器能够起到增加产量和提高能量利用效率的作用。新研发的带有螺旋筛板的新型气升式反应器(airlift reactor with helical sieve plate,ALR-HSP),已经在空气-水实验中证实了其优良的传质特性。为研究反应器综合性能,选取2种具有代表性的真菌(高山被孢霉和毕赤酵母)在ALR-HSP中进行通风培养,分析发酵过程参数和产物组成并与经典气升式反应器(conventional airlift reactor,CALR)进行比较。结果显示:在高山被孢霉培养中,ALR-HSP内菌体的对数生长期的最大比生长速率较CALR提高30%,发酵结束后脂肪酸总量提高约40%;毕赤酵母培养中,ALR-HSP内菌体对数生长期的最大比生长速率较CALR提高20%,发酵结束后菌体浓度增长约20%。且在低通风量条件下,达到相同k L a,ALR-HSP的功率消耗比CALR减少50%。显示出螺旋筛板气升式反应器在耗氧生物培养过程中的优越传质特性和节能效益。High-performance reactors play an important role in increasing yield and energy efficiency in fermentation industries.A novel airlift reactor with helical sieve plate(ALR-HSP)developed in our laboratory had proven its excellent mass transfer characteristics in air-water system.To validate its comprehensive performance,two typical fungi(Mortierella alpine and Pichia pastoris)were selected and cultivated in ALR-HSP comparison to that of conventional airlift reactor(CALR).In the cultivation of M.alpine,the maximal specific growth rate during the logarithmic growth phase of ALR-HSP were 30%higher than that of CALR,and the total fatty acid increased by about 40%under the same culture conditions.,The maximum specific growth rate of P.pastoris during the logarithmic growth phase of ALR-HSP was 20%higher than that of CALR,and the concentration of biomass increased by about 20%.Under the condition of low gas flow rate,the power consumption of ALR-HSP was only about half of CALR when the same k L a was reached.These data show that the ALR-HSP had superior mass transfer characteristics and power saving performance during the process of aerobic biological cultivation.

关 键 词：气升式反应器 高山被孢霉 毕赤酵母 生长速率 比生长速度 氧气消耗速率 

分 类 号：TQ920.5[轻工技术与工程—发酵工程]