HS-SPME-GC-MS法同时测定沙棘酒中11种酯类香气成分  

Determination of 11 ester aroma components in seabuckthorn wine by HS-SPME-GC-MS

作　　者：苗莉莉 刘海丽[2] 惠人杰 MIAO Lili;LIU Haili;HUI Renjie(School of Pharmaceutical Science,Jiangnan University,Wuxi 214122;School of Biotechnology,Jiangnan University,Wuxi 214122)

机构地区：[1]江南大学药学院,无锡214122 [2]江南大学生物工程学院,无锡214122

出　　处：《分析试验室》2020年第8期962-968,共7页Chinese Journal of Analysis Laboratory

摘　　要：建立了顶空固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)联用法同时定量测定沙棘酒中乙酸乙酯、丁酸乙酯、异戊酸乙酯、己酸乙酯、异戊酸异戊酯、庚酸乙酯、乳酸乙酯、辛酸乙酯、苯甲酸乙酯、苯甲酸异戊酯、棕榈酸乙酯的检测方法。选定的顶空固相微萃取最优条件为:50/30μm DVB/CAR/PDMS固相微萃取头、萃取温度40℃、萃取时间40 min、装样量10 mL。采用TG-WAXMS石英毛细管柱,在50℃~230℃的温度范围内梯度升温。各组分在0.001~100 mg/L的范围内均线性关系良好。方法学验证结果显示,各组分的平均回收率为86.3%~109.5%。对市售不同产地沙棘酒中的11种酯类香气成分进行定量分析,准确测定了各产品酯类香气成分的含量,为市售产品的质量评价提供了可靠参考。A method of gas chromatography-mass spectrometry(GC-MS)combined with headspace solid phase microextraction(HS-SPME)was established for simultaneous determination of ethyl acetate,ethyl butyrate,ethyl isovalerate,ethyl hexanoate,isoamyl isovalerate,ethyl heptanoate,ethyl lactate,ethyl octanoate,ethyl benzoate,isoamyl benzoate and ethyl palmitate in seabuckthorn wine.The optimized conditions for HS-SPME were as follows:50/30μm DVB/CAR/PDMS extraction fiber,10 m L of loading volume,and 40 min of extraction at 40℃.The samples were analyzed by TG-WAXMS quartz capillary column(60 m×0.25 mm×0.25μm)and the temperature program raised from 50℃ to 230℃.Under this conditions,all the components showed a good linear relationship in the range of 0.001 to 100 mg/L.The validation of method demonstrated that the precision and repeatability met the requirements,and the average recoveries of all components ranged from 86.3% to 109.5%.The method was then used to quantitatively analyze the ester aroma components in commercial seabuckthorn wine products.

关 键 词：沙棘酒 顶空固相微萃取 气相色谱-质谱 酯类香气成分 定量检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]