基于C14-HSL探讨阪崎肠杆菌中信号分子与其生长机制关系  

Relationship between the Signal Molecule N-Tetradecanoyl-L-homoserine lactone in Cnterobacter sakazakii and Its Growth

作　　者：闫晓彤 罗小娟[1] 潘洁茹 叶海梅 林侃 李长城[1] 方婷[1] YAN Xiaotong;LUO Xiaojuan;PAN Jieru;YE Haimei;LIN Kan;LI Changcheng;FANG Ting(School of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fuzhou Center for Disease Control and Prevention,Fuzhou 350004,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福州市疾病控制预防中心,福建福州350004

出　　处：《食品科学》2020年第18期134-139,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31401597;31601393);福建农林大学国际合作项目(KXb16012A);福建农林大学科技创新专项基金立项项目(KFA17599A;KFA18058A);2018年度福州市卫生计生科研创新团队培育项目(2018-S-wt7);福州市“十三五”海洋经济创新发展示范项目。

摘　　要：研究37℃阪崎克罗诺肠杆菌(Cronobacter sakazakii)CICC21550在生长过程中信号分子C14-HSL浓度变化的拟合曲线,结合细菌生长的宏观模型,进一步从微观角度探究C.sakazakii的生长机制。结果表明:C.sakazakii CICC21550在对数生长初期已检出C14-HSL信号分子,在细菌生长的稳定期达最大值,随后呈逐渐下降趋势,具有明显的密度依赖性。C14-HSL信号分子的最大浓度Ymax为8.535,最大生成速率μmax为0.825。In this paper,the change in the concentration of the signal molecule N-tetradecanoyl-L-homoserine lactone(C14-HSL)during the growth process of Cnterobacter sakazakii CICC21550 at 37℃was fitted to a curve.Based on the macroscopic model of bacterial growth,the growth mechanism of C.sakazakii CICC21550 was further explored from the microscopic perspective.The results showed that C14-HSL was detected in C.akazakii CICC21550 at the early logarithmic phase of growth,which reached the maximum value in the stationary period of bacterial growth,and then showed a gradually declining trend and it was in an obvious density-dependence manner.The maximum concentration Ymax of C14-HSL was 8.535 and the maximum rate of formationμmax was 0.825.

关 键 词：阪崎克罗诺肠杆菌 信号分子 预测模型 IPMP 

分 类 号：TS201.3[轻工技术与工程—食品科学]