苦荞麸皮总黄酮体外抗氧化活性及体内解酒护肝作用  

Antioxidant Activity of Total Flavonoids from Tartary Buckwheat Bran in Vitro and Its Effect on Antialcoholism and Liver Protection in Vivo

作　　者：童钰琴 李姝 牛曼思[2] 王松涛 杨雪琴 孙琴 冉茂芳 TONG Yu-qin;LI Shu;NIU Man-si;WANG Song-tao;YANG Xue-qin;SUN Qin;RAN Mao-fang(School of Pharmacy,Southwest Medical University,Luzhou 646000,China;Luzhou Pinchuang Technology Co.,Ltd.,Luzhou 646000,China;Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry,College of Life Science,Sichuan University,Chengdu 610064,China;Drug Research Center of Integrated Traditional Chinese and Western Medicine,Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]泸州品创科技有限公司,四川泸州646000 [3]四川大学生命科学学院资源微生物学及生物技术教育部重点实验室,四川成都610064 [4]西南医科大学附属中医医院中西医结合药物研究中心,四川泸州646000

出　　处：《食品工业科技》2020年第17期314-319,326,共7页Science and Technology of Food Industry

基　　金：四川省中医药管理局项目(2017C012);泸州市科技计划项目(2016CDLZ-S16、2017-S-49);泸州市科技局项目(2017LZXNYD-T01);西南医大科技项目[2018]6号-51。

摘　　要：目的:研究苦荞麸皮总黄酮(Total Flavonoids from Tartary Buckwheat Bran,TFTB)的体外抗氧化活性及体内解酒护肝作用。方法:在体外抗氧化活性实验中,观察TFTB对超氧阴离子自由基(O2^-·)、DPPH·及NO2^-离子的清除效果。在解酒护肝实验中,将50只小鼠随机分为模型组、阳性药对照组(150 mg/kg·bw)、TFTB低、中、高剂量组(100、200、300 mg/kg·bw)。各组小鼠一次性灌胃溶媒或药物30 min后,再一次性灌胃38%vol浓香型白酒(0.20 mL/10 g),测定小鼠醉酒率、醉酒耐受时间、醉睡时间,以及血清中谷草转氨酶(Aspartate Aminotransferase,AST)和肝脏中丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)水平。结果:TFTB对超氧阴离子自由基(O2^-·)、DPPH·及NO^2-离子均有一定的清除作用,其半抑制浓度(IC50)分别为1.36、0.02、1.79 mg/mL。与醉酒模型组对比,TFTB高剂量组醉酒耐受时间显著延长(P<0.05),TFTB各组醉睡时间极显著缩短(P<0.01);血清中AST含量显著降低(P<0.05),肝组织中SOD活性极显著提高(P<0.01),MDA含量显著降低(P<0.05)。结论:TFTB表现出较强的体外抗氧化活性,具有一定的防醉解酒作用,且在一定程度上能缓解小鼠的酒精性肝损伤。Objective:To study the antioxidant activity in vitro,and relieving alcoholism and hepatoprotective effect in mice by total flavonoids from tartary buckwheat bran(TFTB).Method:In vitro antioxidant experiments,the scavenging effects of TFTB on superoxide anion radical(O2^-·),DPPH·and NO2^- were observed.In the alcohol-relieving and hepatoprotective experiments,fifty mice were randomly divided into drunkenness model group,positive control group(150 mg/kg·bw),TBTF low,medium and high dose group(100,200 and 300 mg/kg·bw).After a one-time gavage of vehicle or drug for 30 min,the mice in each group were given a one-time gavage of Luzhou-flavor liquor of 38%vol(0.20 mL/10 g),and then the drunkenness rate,tolerance time of drunkenness,time of drunkenness and the levels of aspartate aminotransferase(AST)in serum,malondialdehyde(MDA)and superoxide dismutase(SOD)in liver were determined.Results:TFTB had a certain scavenging effect on superoxide anion radical(O2^-·),DPPH·and NO2^-,and the inhibitory concentration 50%(IC50)values were 1.36,0.02 and 1.79 mg/mL.Compared with the model group of drunkenness,the tolerance time of drunkenness in the high dose group of TFTB was significantly prolonged(P<0.05),the groups of TFTB also had a shortened drunkenness(P<0.01),a significant decrease of AST level in serum(P<0.05),and a significant increase SOD activity(P<0.01)and a significant decrease of MDA level in liver(P<0.05).Conclusion:TFTB shows strong antioxidant activity in vitro,and has a certain anti-alcoholic effect and can alleviate alcoholic liver injury in mice to a certain extent.

关 键 词：黄酮 苦荞麸皮 抗氧化活性 醉酒 保肝作用 

分 类 号：TS201.4[轻工技术与工程—食品科学]