基于iTRAQ技术研究锰离子缺乏对植物乳杆菌的影响  

Effect of Manganese Deficiency on Lactobacillus plantarum Based on iTRAQ Technique

作　　者：程新 董英[3] 李昆太[1,2] Cheng Xin;Dong Ying;Li Kuntai(Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,Nanchang 330045;Collage of Biological Science and Engineering,Jiangxi Agricultural University,Nanchang 330045;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,Jiangsu)

机构地区：[1]江西省农业微生物资源开发利用工程实验室,南昌330045 [2]江西农业大学生物科学与工程学院,南昌330045 [3]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《中国食品学报》2020年第9期203-211,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：江苏省优势学科建设项目。

摘　　要：利用iTRAQ技术研究锰离子缺乏对发酵6 h和9 h的植物乳杆菌胞内蛋白表达情况。锰离子缺乏发酵6 h后共鉴定出56种差异蛋白,其中31种蛋白表达上调,25种表达下调。发酵9 h后,共鉴定出78种差异蛋白,其中40种蛋白表达上调,38种蛋白表达下调,共有16种蛋白在2个时间点均表现出显著性差异。锰离子缺乏,乳酸菌胞内多种核糖体结构蛋白的表达量总体下调,而与DNA修复和应激反应相关的蛋白则显著上升,其中UspA家族蛋白是上升最为显著的蛋白之一,说明锰离子缺乏使菌体处于非正常的应激状态。GO和KEGG分析表明:锰离子对丙酮酸代谢、氨基酸代谢等多条代谢通路都有极为显著的影响,说明锰离子是维持乳酸菌正常代谢水平必需的重要因子。Proteomic analysis of Lactobacillus plantarum could provide a theoretical basis for mechanism of manganese involved in metabolism of lactic acid bacteria.Growth of Lactobacillus plantarum DY-2 was observed under the cultivation with or without manganese,and protein expression was analyzed using iTRAQ technology.Bacteria culture were collected at 6 h and 9 h of the mid-logarithmic growth phase for proteomic analysis.Based on iTRAQ results,fifty-six intracellular proteins were identified having differential expression after 6 h with lack of manganese,and these 56 proteins include 31 up-regulated proteins and 25 down-regulated proteins.Seventy-eight intracellular proteins were identified having differential expression after 9 h with lack of manganese,while the expression level of 40 proteins was up-regulated and 38 proteins was down-regulated.Sixteen proteins were found to have significant differences at both time points.Proteins involved in the construction of ribosome were down-regulated with lack of manganese,while the up-regulated proteins were involved in DNA repair and stress response,and UspA family proteins did show significant difference in the fermentation process.GO analysis and KEGG metabolic pathway analysis revealed that manganese have a significant impact on metabolism pathway in L.plantarum,such as pyruvate and amino acid metabolism.

关 键 词：植物乳杆菌 锰离子 ITRAQ 蛋白质组学 

分 类 号：TS201.3[轻工技术与工程—食品科学]