DNA条形码技术在鲑科鱼类真伪鉴别中的应用  

Application of DNA Barcoding Technology for Authentication of Salmonids

作　　者：王楠[1,2] 邢冉冉 马聪聪 乔旭光 陈颖[1] Wang Nan;Xing Ranran;Ma Congcong;Qiao Xuguang;Chen Ying(Chinese Academy of Inspection and Quarantine,Beijing 100176;College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,Shandong)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《中国食品学报》2020年第9期232-240,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2016YFD0401104)。

摘　　要：本研究建立了基于DNA条形码技术的鲑科鱼类特异性检测方法,并用此方法对17种市售鲑科鱼进行物种检测。先从样品中扩增得到DNA细胞色素C氧化酶I(COI)和16S核糖体RNA(16S rRNA)的基因片段,然后进行一代测序,对测序结果进行比对,验证样品的真实属性。结果表明,以COI为靶标、16S rRNA为辅助靶标,可实现对由单一物种组成的鲑鱼制品的物种鉴定。本研究结果证实DNA条形码技术可用于鲑科鱼类的物种鉴别,为鲑科鱼的市场监管提供技术支撑。This study established a DNA barcoding method for the identification of salmonids and then detected 17 commercial salmonid-derived products using this method.The gene fragments of DNA cytochrome C oxidase I(COI)and 16S ribosomal RNA(16S rRNA)were amplified from the samples for Sanger sequencing.The authenticity of the analyzed samples were identified by comparing and analyzing the sequences.For salmonid-derived products that were composed of a single species,the results indicated that species identification could be achieved by using COI as the target and 16S rRNA as the auxiliary target.Thus,our study confirmed DNA barcoding as an effective tool to identify the authenticity of salmonids,which could provide strong technical supports for supervision of salmonids market.

关 键 词：DNA条形码 鲑科鱼类 真伪鉴别 鲑科鱼商品标签 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]