超声辅助提取蓝靛果果实多糖的优化及清除自由基活性研究  

Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Blue Honeysuckle and Scavenging Radicals Activity

作　　者：杨露 郑思韬 鲍元榕 武宝玉 王世杰 徐雅琴[1] YANG Lu;ZHENG Si-tao;BAO Yuan-rong;WU Bao-yu;WANG Shi-jie;XU Ya-qin(College of Art and Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学文理学院,黑龙江哈尔滨150030

出　　处：《食品研究与开发》2020年第20期145-150,共6页Food Research and Development

基　　金：黑龙江省大学生创新创业训练计划项目(201910224081)。

摘　　要：该文在单因素试验的基础上,利用响应面法优化超声波辅助蓝靛果多糖提取工艺。统计分析结果表明影响蓝靛果多糖提取率大小因素依次为:提取时间>超声功率>液料比。最佳提取条件为:时间41 min,液料比为41∶1(mL/g),超声功率310 W。在此条件下,多糖得率为(8.31±0.23)%。清除自由基试验结果表明,蓝靛果多糖对DPPH·、O2-·均有一定的清除作用,最大清除率分别为(53.92±0.88)%和(67.79±1.01)%,半抑制率浓度IC50分别为5.40 mg/mL和0.28 mg/mL。Based on the single factor experiment,the response surface method was used to optimize the extraction process of ultrasonic-assisted extraction polysaccharides from blue honeysuckle(Lonicera caerulea L.)fruits.The statistical analysis indicated that order of effects on the yield of blue honeysuckle polysaccharides:time>ultrasonic power>liquid-to-liquid ratio.The optimal conditions were:extraction time 41 min,liquid-to-liquid ratio 41∶1(mL/g),and ultrasonic power 310 W.Under these conditions,the actual measured yield of polysaccharide was(8.31±0.23)%.The polysaccharide of blue honeysuckle had a certain scavenging effects on DPPH·and O2^-·.The scavenging rates on DPPH·and O2-·were(53.92±0.88)%and(67.79±1.01)%,respectively,and IC50 values were 5.40 mg/mL and 0.28 mg/mL,individually.

关 键 词：蓝靛果 多糖 超声辅助提取 响应面 清除自由基 

分 类 号：TQ281[化学工程—有机化工]