木槿皮提取物的挥发性成分分析及抑菌性研究  

Study on Volatile Components and Antibacterial Activity of Extracts from Bark of Hibiscus syriacus

作　　者：杨靖 王琼波[2] 费嘉祥 杨鹏飞 刘宛凌 李天笑 白冰 毛多斌 YANG Jing;WANG Qiong-bo;FEI Jia-xiang;YANG Peng-fei;LIU Yuan-ling;LI Tian-xiao;BAI Bing;MAO Duo-bin(College of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Department of Food Nutrition,Luohe Medical College,Luohe 462002,China;Xuchang Cigarette Factory,China Tobacco Henan Industrial Co.,Ltd.,Xuchang 461000,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,郑州450002 [2]漯河医学高等专科学校食品营养系,河南漯河462002 [3]河南中烟工业有限责任公司许昌卷烟厂,河南许昌461000

出　　处：《中国调味品》2020年第10期1-5,29,共6页China Condiment

基　　金：2018年河南省科技攻关项目(182102310682)。

摘　　要：以提取率为指标,无水乙醇为溶剂,对超声波萃取木槿皮的影响因素进行了研究,在单因素试验基础上进行正交试验,通过GC×GC-Q-TOFMS对提取物挥发性成分进行分析,并研究了其抑菌性能。试验结果表明,木槿皮提取的最佳工艺条件为液料比15∶1,粉碎度100目,提取时间20 min,提取温度40℃;提取物中共鉴定出41种挥发性成分,占总萃取物的63.78%;主要成分为己二酸-(2-乙基己基)酯(21.35%)、亚油酸(6.25%)、油酸(5.70%);提取物对大肠埃希菌、枯草芽孢杆菌、金黄色葡萄球菌、地衣芽孢杆菌、高地芽孢杆菌、粪肠球菌、肺炎克雷伯氏菌均有一定的抑制作用。Take the extraction rate as the index and anhydrous ethanol as the solvent,the influencing factors of ultrasonic extraction of Hibiscus syriacus bark are studied.Orthogonal test is carried out on the basis of single factor test,the volatile components of the extract are analyzed by GC×GC-QTOFMS,and the antibacterial property is studied.The results show that the optimum process conditions for the extraction of Hibiscus syriacus bark are the liquid-solid ratio of 15∶1,the particle size of crushing of 100 mesh,the extraction time of 20 min,and the extraction temperature of 40℃;there are 41 volatile components identified from the extract,accounting for 63.78%of the total extract;the main components are adipic acid-(2-ethylhexyl)ester(21.35%),linoleic acid(6.25%),oleic acid(5.70%);the extract has certain inhibitory effect on Escherichia coli,Bacillus subtilis,Staphylococcus aureus,Bacillus licheniformis,Bacillus highlands,Enterococcus faecalis and Klebsiella pneumoniae.

关 键 词：木槿皮 提取物 挥发性成分 GC×GC-Q-TOFMS 抑菌性 

分 类 号：TS202.3[轻工技术与工程—食品科学]