发酵小麦胚芽产2,6-二甲氧基对苯醌菌种筛选及发酵条件优化  

Screening and fermentation conditions optimization of 2,6-dimethoxy-ρ-benzoquinone producing strain by fermented wheat germ

作　　者：侯宇豪 常霞 谢秋涛[2] 袁洪燕[2] 刘思含 宋莹 李高阳 HOU Yuhao;CHANG Xia;XIE Qiutao;YUAN Hongyan;LIU Sihan;SONG Ying;LI Gaoyang(Longping Branch Graduate School,Hunan University,Changsha 410125,China;Hunan Agricultural Product Processing Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]湖南大学研究生院隆平分院,湖南长沙410125 [2]湖南省农业科学院农产品加工研究所,湖南长沙410125

出　　处：《中国酿造》2020年第10期125-129,共5页China Brewing

基　　金：长株潭国家自主创新区专项(2018XK2006);湖南省现代农业技术体系(湘财农指(2019)47号)。

摘　　要：该研究以小麦胚芽为原料,2,6-二甲氧基对苯醌(2,6-DMBQ)产量为评价指标,对发酵菌种进行筛选并考察原料预处理方式的影响。在此基础上,采用单因素试验及正交试验对发酵条件进行优化。结果表明,最适发酵菌种为低糖面包酵母;最佳原料预处理方式为超声处理;低糖面包酵母发酵小麦胚芽产2,6-二甲氧基对苯醌的最优发酵条件为菌种添加量1∶8(酵母与麦胚质量比),料液比1∶15(麦胚与水质量比),发酵时间42 h,发酵温度28℃。在此最优发酵条件下,2,6-二甲氧基对苯醌的产量最高为(765.23±9.11)μg/g。The fermentation strains were screened and different pretreatments of raw material were investigated using wheat germ as raw material and 2,6-dimethoxy-ρ-benzoquinone(2,6-DMBQ)as the evaluation index.Based on above tests,the fermentation conditions were optimized by single factor experiments and orthogonal experiments.The results showed that the most suitable fermentation strain and raw material pretreatment was low-sugar baker's yeast and ultrasonic treatment.The optimal fermentation conditions were as follows:strain addition 1∶8(yeast and wheat germ mass ratio),material-liquid ratio 1∶15(wheat germ and water mass ratio),fermentation time 42 h,and temperature 28℃.Under the conditions,the yield of 2,6-dimethoxy-ρ-benzoquinone was(765.23±9.11)μg/g.

关 键 词：小麦胚芽 2 6-二甲氧基对苯醌 菌种筛选 预处理方式 发酵条件 优化 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]